| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRIM36 |
| Uniprot No | Q9NQ86 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-728 aa |
| 活性数据 | MSESGEMSEF GYIMELIAKG KVTIKNIERE LICPACKELF THPLILPCQH SICHKCVKEL LLTLDDSFND VGSDNSNQSS PRLRLPSPSM DKIDRINRPG WKRNSLTPRT TVFPCPGCEH DVDLGERGIN GLFRNFTLET IVERYRQAAR AATAIMCDLC KPPPQESTKS CMDCSASYCN ECFKIHHPWG TIKAQHEYVG PTTNFRPKIL MCPEHETERI NMYCELCRRP VCHLCKLGGN HANHRVTTMS SAYKTLKEKL SKDIDYLIGK ESQVKSQISE LNLLMKETEC NGERAKEEAI THFEKLFEVL EERKSSVLKA IDSSKKLRLD KFQTQMEEYQ GLLENNGLVG YAQEVLKETD QSCFVQTAKQ LHLRIQKATE SLKSFRPAAQ TSFEDYVVNT SKQTELLGEL SFFSSGIDVP EINEEQSKVY NNALINWHHP EKDKADSYVL EYRKINRDDE MSWNEIEVCG TSKIIQDLEN SSTYAFRVRA YKGSICSPCS RELILHTPPA PVFSFLFDEK CGYNNEHLLL NLKRDRVESR AGFNLLLAAE RIQVGYYTSL DYIIGDTGIT KGKHFWAFRV EPYSYLVKVG VASSDKLQEW LRSPRDAVSP RYEQDSGHDS GSEDACFDSS QPFTLVTIGM QKFFIPKSPT SSNEPENRVL PMPTSIGIFL DCDKGKVDFY DMDQMKCLYE RQVDCSHTLY PAFALMGSGG IQLEEPITAK YLEYQEDM |
| 分子量 | 83.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TRIM36蛋白的3篇代表性文献及其摘要概括(信息已简化整理)：
1. **文献名称**：*TRIM36 interacts with the kinetochore protein CENP-H and delays mitotic exit*
**作者**：Li H, et al.
**摘要内容**：研究发现TRIM36通过结合着丝粒蛋白CENP-H参与细胞周期调控，其泛素化活性影响纺锤体检查点功能，异常表达会导致有丝分裂延迟及染色体不稳定性。
2. **文献名称**：*TRIM36 regulates apoptosis via modulating p53 ubiquitination in human osteosarcoma cells*
**作者**：Wang Y, et al.
**摘要内容**：揭示了TRIM36通过泛素化修饰p53调控肿瘤细胞凋亡的机制，为骨肉瘤治疗中靶向TRIM36-p53通路提供潜在策略。
3. **文献名称**：*The E3 ubiquitin ligase TRIM36 modulates sperm head formation by ubiquitinating SPATA16*
**作者**：Zhou Z, et al.
**摘要内容**：首次证明TRIM36在精子顶体形成中的作用，通过泛素化生殖细胞特异蛋白SPATA16影响男性生育能力，为不育症研究提供新方向。
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**注**：以上文献为虚拟简化示例，实际研究中建议通过PubMed或Google Scholar以“TRIM36”为关键词获取最新数据。
Tripartite motif-containing protein 36 (TRIM36) is a member of the TRIM family, characterized by conserved RING, B-box, and coiled-coil domains. Primarily expressed in the testis and brain, it plays roles in ubiquitination, cellular regulation, and developmental processes. Structurally, TRIM36 acts as an E3 ubiquitin ligase, mediating substrate-specific protein degradation via the ubiquitin-proteasome system.
Research highlights its involvement in spermatogenesis, where it stabilizes the sperm head-tail coupling apparatus and regulates acrosome formation. In neurodevelopment, TRIM36 interacts with microtubule-associated proteins, influencing neuronal migration and cortical layer organization. Its dysregulation is linked to male infertility, neurodegenerative disorders, and cancers. For instance, aberrant TRIM36 expression correlates with poor prognosis in glioblastoma and prostate cancer, suggesting tumor-suppressive or oncogenic roles depending on context.
Recombinant human TRIM36 protein, produced via heterologous expression systems, enables functional studies of its enzymatic activity, substrate binding, and interaction networks. Recent studies utilize it to explore mechanisms in DNA damage response, apoptosis, and cell cycle control. However, many aspects of its physiological roles and pathological implications remain unclear, necessitating further research to clarify its therapeutic potential. Its dual involvement in developmental and disease pathways underscores TRIM36 as a multifaceted protein warranting deeper investigation.
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