关键信息
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基因名
RNF43
- 应用
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别名
RNF43；E3 ubiquitin-Protein ligase RNF43
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q68DV7-1
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表达区间
1-197aa
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氨基酸序列
MSGGHQLQLA ALWPWLLMAT LQAGFGRTGL VLAAAVESER SAEQKAIIRV IPLKMDPTGK LNLTLEGVFA GVAEITPAEG KLMQSHPLYL CNASDDDNLE PGFISIVKLE SPRRAPRPCL SLASKARMAG ERGASAVLFD ITEDRAAAEQ LQQPLGLTWP VVLIWGNDAE KLMEFVYKNQ KAHVRIELKE PPAWPDY
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分子量
46 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
RNF43 is an E3 ubiquitin ligase that plays a crucial role in regulating the Wnt signaling pathway, which is essential for various biological processes, including cell proliferation, differentiation, and stem cell maintenance. Aberrant activation of Wnt signaling has been implicated in various cancers, particularly colorectal cancer, making RNF43 a significant focus of research in cancer biology. Studies have demonstrated that mutations and misregulation of RNF43 can lead to the stabilization of Wnt pathway components, promoting tumorigenesis. Furthermore, RNF43 has been identified as a potential tumor suppressor, as its loss of function can result in enhanced Wnt signaling and increased cancer cell proliferation. Researchers have sought to develop recombinant RNF43 protein for various applications, including functional assays to elucidate its role in Wnt signaling modulation and as a potential therapeutic target. The understanding of RNF43's function and the mechanisms governing its activity could lead to novel strategies for cancer treatment and offer insights into the broader implications of Wnt signaling in developmental and disease contexts. As such, the study of RNF43 and its recombinant forms represents a vital area of research with substantial potential for impacting cancer therapeutics and enhancing our understanding of cellular signaling pathways.
