| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRIM29 |
| Uniprot No | Q14134 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-588 aa |
| 活性数据 | MEAADASRSN GSSPEARDAR SPSGPSGSLE NGTKADGKDA KTTNGHGGEA AEGKSLGSAL KPGEGRSALF AGNEWRRPII QFVESGDDKN SNYFSMDSME GKRSPYAGLQ LGAAKKPPVT FAEKGELRKS IFSESRKPTV SIMEPGETRR NSYPRADTGL FSRSKSGSEE VLCDSCIGNK QKAVKSCLVC QASFCELHLK PHLEGAAFRD HQLLEPIRDF EARKCPVHGK TMELFCQTDQ TCICYLCMFQ EHKNHSTVTV EEAKAEKETE LSLQKEQLQL KIIEIEDEAE KWQKEKDRIK SFTTNEKAIL EQNFRDLVRD LEKQKEEVRA ALEQREQDAV DQVKVIMDAL DERAKVLHED KQTREQLHSI SDSVLFLQEF GALMSNYSLP PPLPTYHVLL EGEGLGQSLG NFKDDLLNVC MRHVEKMCKA DLSRNFIERN HMENGGDHRY VNNYTNSFGG EWSAPDTMKR YSMYLTPKGG VRTSYQPSSP GRFTKETTQK NFNNLYGTKG NYTSRVWEYS SSIQNSDNDL PVVQGSSSFS LKGYPSLMRS QSPKAQPQTW KSGKQTMLSH YRPFYVNKGN GIGSNEAP |
| 分子量 | 65.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TRIM29蛋白的模拟参考文献示例(注：文献信息为假设性概括，仅供参考)：
1. **文献名称**：TRIM29 promotes pancreatic cancer progression by stabilizing EZH2 protein
**作者**：Li et al. (2022)
**摘要**：研究发现TRIM29通过泛素化修饰稳定EZH2蛋白，增强胰腺癌细胞侵袭和转移，提示其作为潜在治疗靶点。
2. **文献名称**：TRIM29 regulates DNA damage response via modulation of ATM/ATR signaling
**作者**：Wang et al. (2020)
**摘要**：揭示了TRIM29通过结合ATM/ATR激酶调控DNA损伤修复通路，其缺失导致基因组不稳定性增加，影响癌症化疗敏感性。
3. **文献名称**：Structural basis of TRIM29 B-box domain in antiviral immunity
**作者**：Chen & Smith (2019)
**摘要**：解析了TRIM29 B-box结构域的晶体结构，阐明其通过识别病毒RNA激活先天免疫信号通路的分子机制。
4. **文献名称**：TRIM29 suppresses epithelial-mesenchymal transition in breast cancer by targeting β-catenin
**作者**：Zhang et al. (2021)
**摘要**：发现TRIM29通过促进β-catenin降解抑制乳腺癌EMT过程，低表达TRIM29与患者预后不良相关。
*以上内容为基于TRIM29已知功能的模拟文献，实际文献需通过PubMed/Google Scholar等平台检索确认。*
Tripartite motif-containing protein 29 (TRIM29), also known as ataxia telangiectasia group D-associated protein (ATDC), is a member of the TRIM family characterized by conserved RING, B-box, and coiled-coil domains. Unlike many TRIM proteins, TRIM29 lacks a canonical RING domain with E3 ubiquitin ligase activity, suggesting distinct functional mechanisms. It plays versatile roles in cellular processes, including proliferation, differentiation, apoptosis, innate immunity, DNA damage response, and autophagy. TRIM29 exhibits context-dependent dual roles in cancer, acting as both a tumor suppressor and an oncoprotein. Overexpression is linked to tumor progression in pancreatic, gastric, and bladder cancers, while its downregulation is associated with malignancies like breast and lung cancer. This duality may stem from its interactions with signaling pathways (e.g., p53. Wnt/β-catenin) and epigenetic regulators. TRIM29 localizes to both nuclear and cytoplasmic compartments, influencing its functional diversity. Recombinant TRIM29 protein, commonly produced in bacterial or mammalian expression systems, enables in vitro studies of its structure, post-translational modifications, and biomolecular interactions. Current research focuses on elucidating its structural-functional relationships, particularly how its unique domain architecture mediates protein-protein interactions and nucleic acid binding in pathological conditions. Its involvement in tumorigenesis and therapy resistance highlights TRIM29 as a potential diagnostic biomarker and therapeutic target, though mechanistic insights remain incomplete.
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