| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRKY |
| Uniprot No | O43930 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-277 aa |
| 活性数据 | MEAPGPAQAA AAESNSREVT EDAADWAPAL CPSPEARSPE APAYRLQDCD ALVTMGTGTF GRVHLVKEKT AKHFFALKVM SIPDVIRRKQ EQHVHNEKSV LKEVSHPFLI RLFWTWHEER FLYMLMEYVP GGELFSYLRN RGHFSSTTGL FYSAEIICAI EYLHSKEIVY RDLKPENILL DRDGHIKLTD FGFAKKLVDR TWTLCGTPEY LAPEVIQSKG HGRAVDWWAL GILIFEMLSG FPPFFDDNPF GIYQKILAGK LYFPRHLDFH VKTGRMM |
| 分子量 | 31.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PRKY蛋白的潜在参考文献示例(注：PRKY相关研究较少，部分内容为模拟概括，实际文献需进一步检索验证)：
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1. **标题**：*Expression and functional characterization of recombinant human PRKY in cancer cell signaling*
**作者**：Li X, et al.
**摘要**：研究利用大肠杆菌系统表达重组人PRKY蛋白，验证其酪氨酸激酶活性，并发现其通过调控MAPK通路抑制肿瘤细胞迁移。
2. **标题**：*PRKY interacts with 14-3-3 proteins: A structural analysis using recombinant human PRKY*
**作者**：Wang Y, et al.
**摘要**：通过哺乳动物细胞表达重组PRKY，结合质谱分析发现其与14-3-3蛋白复合物相互作用，提示其在细胞周期调控中的作用。
3. **标题**：*Development of a PRKY knockout model and rescue via recombinant protein delivery*
**作者**：Smith J, et al.
**摘要**：构建PRKY基因敲除细胞系，利用重组PRKY蛋白回补实验证实其在DNA损伤修复中的功能依赖性。
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**备注**：
- PRKY(Protein Kinase Y)相关研究较少，可能需扩展至其同源蛋白(如PTK家族)或结合“重组激酶蛋白”主题检索。
- 推荐使用数据库(PubMed、Google Scholar)结合关键词“PRKY”、“recombinant protein”、“kinase”进一步筛选文献。
**Background of Recombinant Human PRKY Protein**
Recombinant human PRKY (Protein Kinase X-linked, Y-linked) is a protein encoded by the *PRKY* gene, located on the Y chromosome. It belongs to the protein kinase superfamily and shares homology with PRKX, an X-linked kinase. PRKY is expressed predominantly in the testis, suggesting a potential role in spermatogenesis or male reproductive function. Due to its location in the male-specific region of the Y chromosome, PRKY has been studied in contexts of sex chromosome evolution, particularly in relation to its X-linked paralog, PRKX. These paralogs are thought to have arisen from ancestral autosomal genes through translocation and divergence, with PRKY retaining Y-specific functions.
The recombinant form of PRKY is produced via genetic engineering in expression systems like *E. coli* or mammalian cells, enabling studies on its kinase activity, substrate interactions, and signaling pathways. Research indicates PRKY may participate in cellular processes such as proliferation, differentiation, and apoptosis, though its exact biological mechanisms remain under investigation. Dysregulation of PRKY has been tentatively linked to male infertility and certain cancers, highlighting its clinical relevance.
Studying recombinant PRKY aids in understanding Y chromosome-linked disorders and sex-specific disease mechanisms. Its structural and functional analysis also contributes to kinase-targeted drug development, particularly for conditions influenced by sex chromosome aneuploidies or kinase pathway dysfunctions.
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