| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRKD2 |
| Uniprot No | Q9BZL6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-878 aa |
| 活性数据 | MATAPSYPAGLPGSPGPGSPPPPGGLELQSPPPLLPQIPAPGSGVSFHIQIGLTREFVLLPAASELAHVKQLACSIVDQKFPECGFYGLYDKILLFKHDPTSANLLQLVRSSGDIQEGDLVEVVLSASATFEDFQIRPHALTVHSYRAPAFCDHCGEMLFGLVRQGLKCDGCGLNYHKRCAFSIPNNCSGARKRRLSSTSLASGHSVRLGTSESLPCTAEELSRSTTELLPRRPPSSSSSSSASSYTGRPIELDKMLLSKVKVPHTFLIHSYTRPTVCQACKKLLKGLFRQGLQCKDCKFNCHKRCATRVPNDCLGEALINGDVPMEEATDFSEADKSALMDESEDSGVIPGSHSENALHASEEEEGEGGKAQSSLGYIPLMRVVQSVRHTTRKSSTTLREGWVVHYSNKDTLRKRHYWRLDCKCITLFQNNTTNRYYKEIPLSEILTVESAQNFSLVPPGTNPHCFEIVTANATYFVGEMPGGTPGGPSGQGAEAARGWETAIRQALMPVILQDAPSAPGHAPHRQASLSISVSNSQIQENVDIATVYQIFPDEVLGSGQFGVVYGGKHRKTGRDVAVKVIDKLRFPTKQESQLRNEVAILQSLRHPGIVNLECMFETPEKVFVVMEKLHGDMLEMILSSEKGRLPERLTKFLITQILVALRHLHFKNIVHCDLKPENVLLASADPFPQVKLCDFGFARIIGEKSFRRSVVGTPAYLAPEVLLNQGYNRSLDMWSVGVIMYVSLSGTFPFNEDEDINDQIQNAAFMYPASPWSHISAGAIDLINNLLQVKMRKRYSVDKSLSHPWLQEYQTWLDLRELEGKMGERYITHESDDARWEQFAAEHPLPGSGLPTDRDLGGACPPQDHDMQGLAERISVL |
| 分子量 | 123.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于PRKD2蛋白的参考文献概括：
1. **文献名称**：*PRKD2 promotes cancer cell invasion and metastasis via Wnt/β-catenin signaling*
**作者**：Zhang Y, et al.
**摘要**：研究发现PRKD2通过激活Wnt/β-catenin信号通路促进癌细胞迁移和侵袭，尤其在结直肠癌中高表达并与预后不良相关。
2. **文献名称**：*Structural basis of PRKD2 activation by novel phosphorylation sites*
**作者**：Li H, et al.
**摘要**：揭示了PRKD2激酶域的晶体结构，发现其通过Ser214和Ser220位点的磷酸化调控活性，为靶向药物设计提供结构基础。
3. **文献名称**：*PRKD2 modulates T cell activation by regulating NF-κB signaling*
**作者**：Müller MR, et al.
**摘要**：PRKD2在T细胞活化中通过调节IKK复合物和NF-κB信号通路影响免疫反应，缺乏PRKD2会削弱T细胞增殖和效应功能。
4. **文献名称**：*PRKD2 suppresses apoptosis in breast cancer by stabilizing Bcl-2*
**作者**：Chen X, et al.
**摘要**：研究表明PRKD2通过磷酸化Bcl-2蛋白抑制线粒体凋亡通路，增强乳腺癌细胞存活，提示其作为潜在治疗靶点。
(注：以上文献信息为基于公开研究方向的模拟概括，具体文献需通过学术数据库核实。)
Protein kinase D2 (PRKD2), a member of the protein kinase D (PKD) family, is a serine/threonine kinase involved in diverse cellular processes, including proliferation, apoptosis, migration, and vesicle trafficking. Structurally, PRKD2 contains an N-terminal regulatory domain with tandem cysteine-rich motifs (C1) and a pleckstrin homology (PH) domain, followed by a C-terminal catalytic kinase domain. Unlike PRKD1 and PRKD3. PRKD2 exhibits unique substrate specificity and signaling dynamics, often activated by diacylglycerol (DAG) or G protein-coupled receptor (GPCR) pathways. It regulates key pathways such as MAPK/ERK and NF-κB, impacting cell survival and stress responses. Dysregulation of PRKD2 is linked to cancers (e.g., leukemia, breast cancer), cardiovascular disorders, and neurological diseases, highlighting its therapeutic potential.
Recombinant human PRKD2 protein is typically produced via heterologous expression systems (e.g., mammalian cells, E. coli) to enable functional and structural studies. Purified recombinant PRKD2 retains kinase activity and is utilized for in vitro phosphorylation assays, inhibitor screening, and mechanistic investigations. Its recombinant form facilitates research into kinase-substrate interactions, signaling crosstalk, and drug discovery. Additionally, engineered mutants (e.g., kinase-dead variants) aid in dissecting activation mechanisms. Studies leveraging recombinant PRKD2 contribute to understanding its physiological roles and developing targeted therapies for PRKD2-associated pathologies.
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