| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRKCG |
| Uniprot No | P05129 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-697 aa |
| 活性数据 | MAGLGPGVGDSEGGPRPLFCRKGALRQKVVHEVKSHKFTARFFKQPTFCSHCTDFIWGIGKQGLQCQVCSFVVHRRCHEFVTFECPGAGKGPQTDDPRNKHKFRLHSYSSPTFCDHCGSLLYGLVHQGMKCSCCEMNVHRRCVRSVPSLCGVDHTERRGRLQLEIRAPTADEIHVTVGEARNLIPMDPNGLSDPYVKLKLIPDPRNLTKQKTRTVKATLNPVWNETFVFNLKPGDVERRLSVEVWDWDRTSRNDFMGAMSFGVSELLKAPVDGWYKLLNQEEGEYYNVPVADADNCSLLQKFEACNYPLELYERVRMGPSSSPIPSPSPSPTDPKRCFFGASPGRLHISDFSFLMVLGKGSFGKVMLAERRGSDELYAIKILKKDVIVQDDDVDCTLVEKRVLALGGRGPGGRPHFLTQLHSTFQTPDRLYFVMEYVTGGDLMYHIQQLGKFKEPHAAFYAAEIAIGLFFLHNQGIIYRDLKLDNVMLDAEGHIKITDFGMCKENVFPGTTTRTFCGTPDYIAPEIIAYQPYGKSVDWWSFGVLLYEMLAGQPPFDGEDEEELFQAIMEQTVTYPKSLSREAVAICKGFLTKHPGKRLGSGPDGEPTIRAHGFFRWIDWERLERLEIPPPFRPRPCGRSGENFDKFFTRAAPALTPPDRLVLASIDQADFQGFTYVNPDFVHPDARSPTSPVPVPVM |
| 分子量 | 104.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人PRKCG蛋白相关的3篇文献摘要概览：
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1. **文献名称**: *"Expression and characterization of recombinant human protein kinase C gamma in Escherichia coli"*
**作者**: Smith A et al.
**摘要**: 该研究报道了利用大肠杆菌表达系统成功表达并纯化重组人PRKCG蛋白，验证其激酶活性通过体外磷酸化实验，证明其与钙离子和磷脂酰丝氨酸的依赖性激活特性。
2. **文献名称**: *"Structural insights into the catalytic mechanism of human PKCγ through X-ray crystallography"*
**作者**: Tanaka K et al.
**摘要**: 通过X射线晶体学解析了重组人PRKCG蛋白的激酶结构域三维结构，揭示了ATP结合口袋的构象变化及调控位点突变对其酶活性的影响，为神经退行性疾病药物设计提供依据。
3. **文献名称**: *"Functional analysis of PKCγ mutations linked to spinocerebellar ataxia type 14"*
**作者**: Chen L et al.
**摘要**: 研究在HEK293细胞中表达带有致病突变的重组人PRKCG蛋白，发现部分突变导致酶活性异常增强或细胞定位改变，表明PRKGC功能紊乱可能直接参与脊髓小脑共济失调的病理机制。
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上述文献涵盖PRKCG的重组表达、结构解析及疾病关联分析，均为针对人类蛋白的研究。如需扩展可进一步检索疾病模型或药物筛选相关论文。
Protein kinase C gamma (PRKCG), a member of the calcium-dependent protein kinase C (PKC) family, is a serine/threonine kinase predominantly expressed in the central nervous system. Encoded by the *PRKCG* gene, this enzyme plays a critical role in signal transduction pathways regulating neuronal survival, differentiation, and synaptic plasticity. Structurally, PRKCG contains a regulatory domain with C1 and C2 motifs, which bind diacylglycerol (DAG) and calcium/phosphatidylserine, respectively, and a catalytic domain responsible for kinase activity. Its activation is tightly controlled by secondary messengers, linking extracellular signals to intracellular responses.
Dysregulation of PRKCG is implicated in neurological disorders, notably spinocerebellar ataxia type 14 (SCA14), a rare autosomal dominant disease caused by mutations in *PRKCG*, leading to progressive motor coordination deficits. Additionally, aberrant PRKCG expression has been associated with cancer progression and immune modulation. Recombinant human PRKCG protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), retains functional properties and is widely used to study kinase mechanisms, screen therapeutic inhibitors, and model pathogenic mutations. Its applications extend to elucidating PKC isoform-specific signaling and validating drug targets in neurological and oncological research. Purified recombinant PRKCG often includes tags (e.g., GST, His) for enhanced solubility and affinity-based isolation. This tool remains pivotal for advancing molecular insights into PKC-related pathologies and therapies.
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