| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRKCZ |
| Uniprot No | P17252 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-672 aa |
| 活性数据 | ADVFPGNDS TASQDVANRF ARKGALRQKN VHEVKDHKFI ARFFKQPTFC SHCTDFIWGF GKQGFQCQVC CFVVHKRCHE FVTFSCPGAD KGPDTDDPRS KHKFKIHTYG SPTFCDHCGS LLYGLIHQGM KCDTCDMNVH KQCVINVPSL CGMDHTEKRG RIYLKAEVAD EKLHVTVRDA KNLIPMDPNG LSDPYVKLKL IPDPKNESKQ KTKTIRSTLN PQWNESFTFK LKPSDKDRRL SVEIWDWDRT TRNDFMGSLS FGVSELMKMP ASGWYKLLNQ EEGEYYNVPI PEGDEEGNME LRQKFEKAKL GPAGNKVISP SEDRKQPSNN LDRVKLTDFN FLMVLGKGSF GKVMLADRKG TEELYAIKIL KKDVVIQDDD VECTMVEKRV LALLDKPPFL TQLHSCFQTV DRLYFVMEYV NGGDLMYHIQ QVGKFKEPQA VFYAAEISIG LFFLHKRGII YRDLKLDNVM LDSEGHIKIA DFGMCKEHMM DGVTTRTFCG TPDYIAPEII AYQPYGKSVD WWAYGVLLYE MLAGQPPFDG EDEDELFQSI MEHNVSYPKS LSKEAVSVCK GLMTKHPAKR LGCGPEGERD VREHAFFRRI DWEKLENREI QPPFKPKVCG KGAENFDKFF TRGQPVLTPP DQLVIANIDQ SDFEGFSYVN PQFVHPILQS AV |
| 分子量 | 76.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于重组人 **PRKCZ(Protein Kinase C Zeta)** 蛋白的研究文献示例及简要摘要：
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1. **文献名称**：*"Functional characterization of recombinant human PKCζ in kinase signaling pathways"*
**作者**：Leitges M, et al.
**摘要**：该研究成功表达并纯化了重组人PRKCZ蛋白，验证其体外激酶活性，证明其在PI3K/Akt和NF-κB信号通路中的关键调控作用，并通过突变实验揭示其催化结构域的重要性。
2. **文献名称**：*"Structural insights into the autoinhibition mechanism of atypical protein kinase C zeta"*
**作者**：Takashi T, et al.
**摘要**：通过X射线晶体学解析了重组PRKCZ蛋白的全长结构，发现其PB1结构域与激酶结构域的自抑制相互作用，为设计靶向PRKCZ的调控分子提供结构基础。
3. **文献名称**：*"PRKCZ interacts with 14-3-3 proteins to regulate cancer cell survival under oxidative stress"*
**作者**：Chen L, et al.
**摘要**：利用重组PRKCZ蛋白进行互作实验，发现其通过磷酸化依赖方式与14-3-3蛋白结合，维持肿瘤细胞在氧化应激下的存活能力，为癌症治疗提供潜在靶点。
4. **文献名称**：*"High-yield production of recombinant human PRKCZ in Escherichia coli and its application in drug screening"*
**作者**：Kim S, Park JH
**摘要**：报道了一种优化的大肠杆菌表达系统，高效制备具有生物活性的重组PRKCZ蛋白，并用于高通量筛选抑制其激酶活性的小分子化合物。
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**注**：以上文献标题及内容为模拟概括，实际研究需查阅具体论文(可通过PubMed、Web of Science检索关键词：**PKCζ、PRKCZ、recombinant protein**)。
**Background of Recombinant Human PRKCZ Protein**
Protein kinase C zeta (PRKCZ), a member of the protein kinase C (PKC) family, is a serine/threonine kinase involved in diverse cellular processes, including cell proliferation, survival, and polarity. Unlike classical PKC isoforms, PRKCZ belongs to the atypical PKC subfamily, which lacks calcium dependency and diacylglycerol sensitivity. It is activated by phosphoinositide-dependent signaling pathways, particularly through interactions with phosphatidylinositol 3-kinase (PI3K) and small GTPases like Rac1/Cdc42. Structurally, PRKCZ contains a kinase domain and a regulatory region with a PB1 domain that facilitates protein-protein interactions.
PRKCZ plays critical roles in signaling pathways such as NF-κB activation, MAPK/ERK cascades, and insulin-responsive glucose transport. It is implicated in cellular responses to oxidative stress, immune regulation, and neuronal development. Dysregulation of PRKCZ is associated with pathologies like cancer (e.g., tumor invasion, drug resistance), metabolic disorders (e.g., diabetes), and neurodegenerative diseases.
Recombinant human PRKCZ protein, produced via molecular cloning in bacterial or eukaryotic expression systems, retains functional kinase activity and is widely used to study its enzymatic mechanisms, signaling networks, and therapeutic targeting. Purification methods, such as affinity chromatography, ensure high specificity. Research applications include in vitro kinase assays, protein interaction studies, and drug screening. Validated by techniques like SDS-PAGE and Western blotting, recombinant PRKCZ provides a vital tool for dissecting its physiological and pathological roles.
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