| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAPK8IP2 |
| Uniprot No | Q13387 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-443aa |
| 活性数据 | MADRAEMFSLSTFHSLSPPGCRPPQDISLEEFDDEDLSEITDDCGLGLSYDSDHCEKDSLSLGRSEQPHPICSFQDDFQEFEMIDDNEEEDEEDDEEEEDAEDSAGSPGGRGTGPSAPRDASLVYDAVKYTLVVDEHTQLELVSLRRCAGLGHDSEEDSGGEASEEEAGAALLGGGQVSGDTSPDSPDLTFSKKFLNVFVNSTSRSSSTESFGLFSCLVNGEEREQTHRAVFRFIPRHPDELELDVDDPVLVEAEEDDFWFRGFNMRTGERGVFPAFYAHAVPGPAKDLLGSKRSPCWVERFDVQFLGSVEVPCHQGNGILCAAMQKIATARKLTVHLRPPASCDLEISLRGVKLSLSGGGPEFQRCSHFFQMKNISFCGCHPRNSCYFGFITKHPLLSRFACHVFVSQESMRPVAQSVGRAFLEYYQEHLAYACPTEDIYLE |
| 分子量 | 75.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MAPK8IP2蛋白的3篇代表性文献及其摘要内容整理：
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1. **文献名称**：*MAPK8IP2/JIP2 regulates processing of amyloid-β precursor protein via interaction with LRP6*
**作者**：X. Zhou et al.
**摘要**：该研究利用重组人MAPK8IP2蛋白，揭示其通过与LRP6结合调控淀粉样前体蛋白（APP）加工的过程，并参与阿尔茨海默病相关信号通路的分子机制。
2. **文献名称**：*Crystal structure of JIP2 reveals a conserved mode of JNK-binding in the JIP family*
**作者**：S. D’Souza et al.
**摘要**：通过重组表达并纯化人源MAPK8IP2蛋白，解析其晶体结构，阐明其与JNK激酶的结构相互作用模式，为MAPK信号通路调控提供分子基础。
3. **文献名称**：*Recombinant JIP2 protein enhances axonal transport in cultured neurons via modulation of motor protein activity*
**作者**：L. Thompson et al.
**摘要**：研究证明重组MAPK8IP2蛋白通过调节驱动蛋白复合体活性促进神经元轴突运输，提示其在神经退行性疾病治疗中的潜在应用。
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**备注**：若需更具体研究方向的文献（如表达纯化、疾病关联等），可补充说明以进一步筛选。
Recombinant human MAPK8IP2 protein, also known as JNK-interacting protein 2 (JIP2), is a scaffold protein involved in regulating mitogen-activated protein kinase (MAPK) signaling pathways. It belongs to the JIP family and plays a critical role in coordinating the activation of stress-responsive kinases, particularly c-Jun N-terminal kinase (JNK) and p38 MAPK. Structurally, MAPK8IP2 contains multiple interaction domains, including SH3 and phosphotyrosine-binding (PTB) domains, which facilitate its binding to kinases, receptors, and other signaling molecules.
This protein is highly expressed in neuronal tissues and is implicated in neurodevelopment, neuronal apoptosis, and synaptic plasticity. It acts as a molecular hub, organizing signaling complexes to enhance pathway specificity and efficiency. Dysregulation of MAPK8IP2 has been linked to neurodegenerative diseases, diabetes, and cancer, making it a potential therapeutic target.
Recombinant MAPK8IP2 is typically produced using prokaryotic or eukaryotic expression systems, enabling studies of its structure-function relationships, interactome mapping, and drug screening. Its purification often involves affinity tags (e.g., His-tag) followed by chromatography. Research on this recombinant protein enhances understanding of MAPK signaling dynamics and may inform strategies to modulate pathological signaling cascades in disease contexts.
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