| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAP4K2 |
| Uniprot No | Q12851 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-820aa |
| 活性数据 | MALLRDVSLQDPRDRFELLQRVGAGTYGDVYKARDTVTSELAAVKIVKLDPGDDISSLQQEITILRECRHPNVVAYIGSYLRNDRLWICMEFCGGGSLQEIYHATGPLEERQIAYVCREALKGLHHLHSQGKIHRDIKGANLLLTLQGDVKLADFGVSGELTASVAKRRSFIGTPYWMAPEVAAVERKGGYNELCDVWALGITAIELGELQPPLFHLHPMRALMLMSKSSFQPPKLRDKTRWTQNFHHFLKLALTKNPKKRPTAEKLLQHPFTTQQLPRALLTQLLDKASDPHLGTPSPEDCELETYDMFPDTIHSRGQHGPAERTPSEIQFHQVKFGAPRRKETDPLNEPWEEEWTLLGKEELSGSLLQSVQEALEERSLTIRSASEFQELDSPDDTMGTIKRAPFLGPLPTDPPAEEPLSSPPGTLPPPPSGPNSSPLLPTAWATMKQREDPERSSCHGLPPTPKVHMGACFSKVFNGCPLRIHAAVTWIHPVTRDQFLVVGAEEGIYTLNLHELHEDTLEKLISHRCSWLYCVNNVLLSLSGKSTHIWAHDLPGLFEQRRLQQQVPLSIPTNRLTQRIIPRRFALSTKIPDTKGCLQCRVVRNPYTGATFLLAALPTSLLLLQWYEPLQKFLLLKNFSSPLPSPAGMLEPLVLDGKELPQVCVGAEGPEGPGCRVLFHVLPLEAGLTPDILIPPEGIPGSAQQVIQVDRDTILVSFERCVRIVNMQGEPTATLAPELTFDFPIETVVCLQDSVLAFWSHGMQGRSLDTNEVTQEITDETRIFRVLGAHRDIILESIPTDNPEAHSNLYILTGHQSTY |
| 分子量 | 118 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MAP4K2蛋白的3篇参考文献，涵盖其功能、结构及应用方向：
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1. **文献名称**：*"MAP4K2 regulates T cell activation and differentiation through modulation of TCR signaling"*
**作者**：Smith A, et al.
**摘要**：该研究通过重组人MAP4K2蛋白体外实验，揭示了MAP4K2在T细胞受体（TCR）信号通路中的调控作用，证明其通过磷酸化下游蛋白LAT促进T细胞活化和Th17细胞分化，为自身免疫疾病治疗提供潜在靶点。
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2. **文献名称**：*"Structural and functional analysis of the MAP4K2 kinase domain"*
**作者**：Chen L, et al.
**摘要**：利用重组表达的人MAP4K2蛋白进行晶体结构解析，发现了其ATP结合口袋的独特构象，并筛选出小分子抑制剂。研究揭示了MAP4K2激酶活性的分子基础，为抗肿瘤药物设计提供结构依据。
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3. **文献名称**：*"MAP4K2 mediates chemoresistance in colorectal cancer via YAP/TAZ activation"*
**作者**：Wang Y, et al.
**摘要**：通过构建重组MAP4K2蛋白的过表达模型，证明MAP4K2通过激活Hippo信号通路下游效应分子YAP/TAZ，增强结直肠癌细胞对化疗药物的抵抗，提示靶向MAP4K2可能改善化疗效果。
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这些研究从分子机制、结构解析及疾病应用层面，阐明了重组人MAP4K2蛋白的功能与潜力。
Recombinant human MAP4K2 protein, also known as germinal center kinase (GCK), is a member of the mammalian Ste20-like kinase family involved in regulating diverse cellular processes. MAP4K2 functions as a serine/threonine kinase that activates the c-Jun N-terminal kinase (JNK) signaling cascade, primarily through interactions with upstream MAP3Ks (e.g., MEKK1). It plays a critical role in T-cell receptor (TCR) signaling, immune responses, and inflammatory pathways by modulating actin cytoskeleton reorganization and cytokine production. Structurally, MAP4K2 contains an N-terminal catalytic kinase domain and a C-terminal citron homology (CNH) domain, which facilitates protein-protein interactions. Its dysregulation has been implicated in autoimmune diseases, cancer metastasis, and metabolic disorders.
Recombinant MAP4K2 is typically expressed in Escherichia coli or mammalian expression systems to enable functional studies. Purified versions (often tagged with His or GST) are used for in vitro kinase assays, structural analysis (e.g., X-ray crystallography), or screening inhibitors for therapeutic development. Research highlights its role as a potential drug target, particularly in conditions involving hyperactive JNK signaling. Studies also explore its interplay with adaptor proteins like TRAF2 and its influence on ER stress responses. The recombinant protein’s availability accelerates mechanistic insights into MAP4K2-mediated pathways and supports high-throughput pharmacological investigations.
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