| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAP4K4 |
| Uniprot No | O95819 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-289aa |
| 活性数据 | FELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFI |
| 分子量 | 0 |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MAP4K4蛋白的模拟参考文献示例（内容为虚构，供参考）：
1. **标题**: *Structural and functional characterization of recombinant human MAP4K4 kinase domain*
**作者**: Smith A, et al.
**摘要**: 该研究通过大肠杆菌系统表达并纯化了重组人MAP4K4激酶结构域，利用X射线晶体学解析其三维结构。功能实验表明该重组蛋白具有ATP依赖性激酶活性，并验证了其在调控JNK信号通路中的作用。
2. **标题**: *MAP4K4 knockdown inhibits tumor metastasis via modulation of recombinant protein-mediated integrin signaling*
**作者**: Chen L, et al.
**摘要**: 研究利用重组人MAP4K4蛋白进行体外激酶活性分析，发现其通过磷酸化下游靶点促进肿瘤细胞迁移和侵袭。实验进一步显示，抑制MAP4K4活性可阻断整合素信号通路，降低转移风险。
3. **标题**: *Development of a high-throughput screening assay for MAP4K4 inhibitors using recombinant protein*
**作者**: Johnson R, et al.
**摘要**: 作者建立了一种基于重组人MAP4K4蛋白的高通量激酶活性检测方法，用于筛选小分子抑制剂。研究成功鉴定出多个具有纳摩尔级抑制活性的化合物，为治疗炎症性疾病提供了潜在药物先导结构。
4. **标题**: *Recombinant MAP4K4 modulates T cell activation and cytokine production in autoimmune models*
**作者**: Wang Y, et al.
**摘要**: 本文通过体外实验证明，重组人MAP4K4蛋白可增强T细胞受体信号传导，促进IL-17等促炎因子分泌。该发现揭示了MAP4K4在自身免疫疾病中的调控机制，为靶向治疗提供了理论依据。
**注**: 以上文献信息为模拟生成，实际引用需查询真实数据库（如PubMed）。如需真实文献，建议通过关键词 *"recombinant human MAP4K4"* 或 *"MAP4K4 kinase activity"* 在学术平台检索。
Recombinant human MAP4K4 (mitogen-activated protein kinase kinase kinase kinase 4) is a protein of significant interest in cellular signaling research. As a member of the Ste20 family of serine/threonine kinases, MAP4K4 plays a crucial role in regulating stress-activated pathways, including the c-Jun N-terminal kinase (JNK) and p38 MAPK cascades. Structurally, it contains an N-terminal kinase domain and a C-terminal regulatory region, which facilitate interactions with adaptor proteins and downstream effectors. MAP4K4 is implicated in diverse physiological and pathological processes, such as inflammation, immune response, apoptosis, cell migration, and insulin resistance. Dysregulation of MAP4K4 has been linked to metabolic disorders, cancer metastasis, and cardiovascular diseases, making it a potential therapeutic target.
The recombinant form of MAP4K4 is typically produced using eukaryotic expression systems (e.g., HEK293 or insect cells) or prokaryotic systems (e.g., E. coli) to ensure proper folding and post-translational modifications. Purification often involves affinity chromatography tags (e.g., His-tag) for high yield and purity. Recombinant MAP4K4 enables researchers to study its enzymatic activity, substrate specificity, and inhibitor screening in vitro. Recent studies highlight its role in tumor microenvironment modulation and metabolic reprogramming, driving interest in developing small-molecule inhibitors. Challenges remain in understanding isoform-specific functions and tissue-dependent signaling nuances. Overall, recombinant MAP4K4 serves as a vital tool for dissecting kinase-mediated pathways and advancing targeted therapies.
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