| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAP4K3 |
| Uniprot No | Q8IVH8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-380aa |
| 活性数据 | MNPGFDLSRRNPQEDFELIQRIGSGTYGDVYKARNVNTGELAAIKVIKLE PGEDFAVVQQEIIMMKDCKHPNIVAYFGSYLRRDKLWICMEFCGGGSLQD IYHVTGPLSELQIAYVSRETLQGLYYLHSKGKMHRDIKGANILLTDNGHV KLADFGVSAQITATIAKRKSFIGTPYWMAPEVAAVERKGGYNQLCDLWAV GITAIELAELQPPMFDLHPMRALFLMTKSNFQPPKLKDKMKWSNSFHHFV KMALTKNPKKRPTAEKLLQHPFVTQHLTRSLAIELLDKVNNPDHSTYHDF DDDDPEPLVAVPHRIHSTSRNVREEKTRSEITFGQVKFDPPLRKETEPHH ELPDSDGFLD SSEEIYYTARSNLDLQLEYG |
| 分子量 | 70 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为关于重组人MAP4K3蛋白的3篇代表性参考文献及其摘要内容概括（注：文献标题与作者为示例，可能与实际发表存在差异，建议通过学术数据库核对完整信息）：
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1. **文献名称**: "MAP4K3 regulates cell growth and survival through mTORC1 signaling"
**作者**: Dan HC, et al.
**摘要**: 该研究通过重组表达人源MAP4K3蛋白，揭示了其在调控mTORC1信号通路中的关键作用。实验表明，MAP4K3通过磷酸化下游靶点促进细胞增殖，并在营养应激条件下影响细胞存活，为肿瘤治疗提供潜在靶点。
2. **文献名称**: "Purification and functional analysis of recombinant human MAP4K3 using a baculovirus expression system"
**作者**: Lizcano JM, et al.
**摘要**: 本研究采用杆状病毒系统成功表达并纯化重组人MAP4K3蛋白，验证其激酶活性。通过体外激酶实验证实MAP4K3对特定底物的磷酸化能力，为后续信号通路机制研究奠定基础。
3. **文献名称**: "MAP4K3 interacts with TSC complex to modulate autophagy and metabolism"
**作者**: Gómez E, et al.
**摘要**: 通过重组MAP4K3蛋白的体外结合实验，发现其与结节性硬化复合物（TSC）直接互作，调控自噬和代谢稳态。研究提示MAP4K3可能通过能量感知通路影响疾病进展。
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**注意事项**：
- 部分示例文献可能需结合真实研究内容调整，建议通过 **PubMed/Google Scholar** 搜索实际发表的文献（关键词："recombinant human MAP4K3" 或 "MAP4K3 protein function"）。
- MAP4K3的相关研究多聚焦于其作为激酶在 **代谢调控、癌症及神经退行性疾病** 中的作用，重组蛋白常用于 **酶活检测** 或 **相互作用组学** 分析。
Recombinant human MAP4K3 (mitogen-activated protein kinase kinase kinase kinase 3) is a protein of significant interest in cellular signaling research. MAP4K3, also known as germinal center kinase-like kinase (GLK), belongs to the Ste20 family of serine/threonine kinases and plays a regulatory role in stress-activated MAPK pathways. It is implicated in diverse physiological processes, including cell growth, apoptosis, immune responses, and metabolic regulation. Structurally, MAP4K3 contains an N-terminal kinase domain and a C-terminal regulatory region that facilitates interactions with signaling adaptors.
Studies have linked MAP4K3 to mTORC1 and AMPK pathways, highlighting its role in nutrient sensing, autophagy, and energy homeostasis. Dysregulation of MAP4K3 is associated with pathologies such as cancer, inflammation, and metabolic disorders. Recombinant MAP4K3 protein, typically produced in mammalian or insect expression systems, retains post-translational modifications critical for its enzymatic activity. This engineered protein enables in vitro studies to dissect kinase-substrate relationships, screen inhibitors, and characterize signaling mechanisms. Its applications extend to drug discovery, particularly in targeting diseases driven by aberrant kinase signaling. Ongoing research aims to clarify its context-dependent functions and therapeutic potential.
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