| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAPK4 |
| Uniprot No | P31152 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-587aa |
| 活性数据 | MAEKGDCIASVYGYDLGGRFVDFQPLGFGVNGLVLSAVDSRACRKVAVKKIALSDARSMKHALREIKIIRRLDHDNIVKVYEVLGPKGTDLQGELFKFSVAYIVQEYMETDLARLLEQGTLAEEHAKLFMYQLLRGLKYIHSANVLHRDLKPANIFISTEDLVLKIGDFGLARIVDQHYSHKGYLSEGLVTKWYRSPRLLLSPNNYTKAIDMWAAGCILAEMLTGRMLFAGAHELEQMQLILETIPVIREEDKDELLRVMPSFVSSTWEVKRPLRKLLPEVNSEAIDFLEKILTFNPMDRLTAEMGLQHPYMSPYSCPEDEPTSQHPFRIEDEIDDIVLMAANQSQLSNWDTCSSRYPVSLSSDLEWRPDRCQDASEVQRDPRAGSAPLAEDVQVDPRKDSHSSSERFLEQSHSSMERAFEADYGRSCDYKVGSPSYLDKLLWRDNKPHHYSEPKLILDLSHWKQAAGAPPTATGLADTGAREDEPASLFLEIAQWVKSTQGGPEHASPPADDPERRLSASPPGRPAPVDGGASPQFDLDVFISRALKLCTKPEDLPDNKLGDLNGACIPEHPGDLVQTEAFSKERW |
| 分子量 | 64.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MAPK4蛋白的虚构参考文献示例，供参考：
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1. **文献名称**: *Recombinant Human MAPK4 Expression and Its Role in Cancer Cell Apoptosis*
**作者**: Smith, J. et al.
**摘要**: 该研究通过大肠杆菌系统成功表达并纯化重组人MAPK4蛋白，发现其通过抑制caspase-3活性促进癌细胞增殖，并增强肿瘤细胞对化疗药物的耐药性，提示MAPK4可能是癌症治疗的潜在靶点。
2. **文献名称**: *Crystal Structure of Recombinant MAPK4 Reveals Unique Activation Loop Dynamics*
**作者**: Johnson, R. & Lee, S.
**摘要**: 本文首次解析了重组人MAPK4的晶体结构，发现其激活环（activation loop）的构象变化不同于其他MAPK家族成员，揭示了其非典型磷酸化调控机制，为设计特异性抑制剂提供结构基础。
3. **文献名称**: *Recombinant MAPK4 Modulates Inflammatory Signaling in Macrophages*
**作者**: Chen, L. et al.
**摘要**: 研究利用哺乳动物细胞表达的重组MAPK4，证明其通过负调控NF-κB通路抑制巨噬细胞中促炎因子（如TNF-α、IL-6）的分泌，提示MAPK4在炎症性疾病中的调控作用。
4. **文献名称**: *MAPK4 Knockdown via siRNA and Recombinant Protein Rescue in Neuronal Development*
**作者**: Wang, Y. et al.
**摘要**: 通过siRNA敲低MAPK4并结合重组蛋白回补实验，发现MAPK4对神经元突触形成至关重要，其功能依赖于与下游靶蛋白PSD-95的相互作用。
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注：以上内容为模拟示例，实际文献需通过学术数据库（如PubMed、Web of Science）检索。如需真实文献，建议查询关键词 *"recombinant human MAPK4"* 或 *"MAPK4 protein function"*。
Recombinant human MAPK4 (Mitogen-Activated Protein Kinase 4) is a engineered version of the endogenous serine/threonine-protein kinase involved in critical cellular signaling pathways. MAPK4, a less-studied member of the MAPK family, plays regulatory roles in processes such as cell proliferation, differentiation, stress response, and apoptosis. Unlike classical MAPKs (e.g., ERK, p38, JNK), MAPK4 lacks a conserved TXY activation motif, suggesting distinct activation mechanisms. It is implicated in diverse physiological and pathological contexts, including cancer progression, neurodegenerative diseases, and immune regulation.
The recombinant form is typically produced using heterologous expression systems (e.g., E. coli, insect, or mammalian cells) to enable high-purity, functional protein production for research. Researchers utilize recombinant MAPK4 to study its structure, substrate specificity, and interaction partners, often focusing on its atypical signaling cascades. Its role in modulating mTORC1 signaling and promoting cell survival under stress has drawn attention in oncology and neurobiology. However, its precise molecular mechanisms remain incompletely defined, driving demand for recombinant tools to resolve its biological functions.
Studies leveraging recombinant MAPK4 aim to clarify its dual role in both tumor suppression and pro-survival signaling, potentially informing therapeutic strategies. Its commercial availability accelerates target validation and inhibitor screening, addressing gaps in MAPK pathway understanding.
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