| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAP6D1 |
| Uniprot No | Q9H9H5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-199aa |
| 活性数据 | MAWPCISRLCCLARRWNQLDRSDVAVPLTLHGYSDLDSEEPGTGGAASRRGQPPAGARDSGRDVPLTQYQRDFGLWTTPAGPKDPPPGRGPGAGGRRGKSSAQSSAPPAPGARGVYVLPIGDADAAAAVTTSYRQEFQAWTGVKPSRSTKTKPARVITTHTSGWDSSPGAGFQVPEVRKKFTPNPSAIFQASAPRILNV |
| 分子量 | 47.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MAP6D1蛋白的虚构参考文献示例，基于相关研究领域的常见方向总结：
---
1. **文献名称**: "Structural and Functional Characterization of Recombinant Human MAP6D1 Protein"
**作者**: Smith J. et al.
**摘要**: 通过大肠杆菌表达系统成功纯化重组人MAP6D1蛋白，并解析其晶体结构。研究表明MAP6D1通过N端结构域结合微管，并在体外实验中稳定微管网络，提示其在神经元细胞骨架动态调节中的潜在作用。
2. **文献名称**: "MAP6D1 Deficiency Alters Neuronal Morphogenesis in Cortical Neurons"
**作者**: Chen L. et al.
**摘要**: 利用CRISPR技术敲除MAP6D1基因，发现小鼠皮质神经元树突分支减少。通过重组MAP6D1蛋白回补实验证实其通过调控微管稳定性促进突触形成，暗示该蛋白与神经发育障碍相关。
3. **文献名称**: "Recombinant MAP6D1 Binds to Tubulin with High Affinity: Insights from SPR Analysis"
**作者**: García R. et al.
**摘要**: 采用表面等离子体共振（SPR）技术测定重组MAP6D1与微管蛋白的相互作用，发现结合亲和力（KD≈50nM）。竞争实验表明其结合位点与经典微管稳定剂Taxol部分重叠，为开发神经保护剂提供理论依据。
4. **文献名称**: "MAP6D1 Expression is Downregulated in Schizophrenia Postmortem Brains"
**作者**: Tanaka K. et al.
**摘要**: 通过分析精神分裂症患者死后脑组织，发现前额叶皮层MAP6D1 mRNA及蛋白水平显著降低。体外实验显示重组MAP6D1可逆转神经元突触可塑性异常，提示其表达缺陷可能与疾病病理相关。
---
注：以上内容为模拟文献概括，实际研究中需依据具体文献进行引用。建议通过PubMed等数据库以“MAP6D1 recombinant protein”为关键词检索最新实证研究。
**Background of Recombinant Human MAP6D1 Protein**
Recombinant human MAP6D1 (Microtubule-Associated Protein 6 Domain-Containing 1) is a engineered protein derived from the human *MAP6D1* gene, which encodes a member of the microtubule-associated protein family. MAP6D1 is involved in microtubule dynamics, playing a critical role in stabilizing microtubules and regulating cytoskeletal organization, essential for cellular processes like cell division, intracellular transport, and neuronal development. Structurally, MAP6D1 contains conserved domains that mediate interactions with microtubules and other cytoskeletal components, influencing their polymerization and stability.
The recombinant form is typically produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to ensure high purity and functionality for research applications. Studies suggest MAP6D1 may contribute to neurodevelopmental and neuropsychiatric disorders, as disrupted microtubule dynamics are linked to aberrant synaptic plasticity and neuronal connectivity. Additionally, its role in cancer is being explored, given the reliance of cancer cells on microtubule remodeling for proliferation and metastasis.
Research on recombinant MAP6D1 enables mechanistic insights into these pathways, aiding drug discovery and the development of biomarkers. However, its full interactome and regulatory mechanisms remain under investigation, necessitating further studies to elucidate its physiological and pathological significance.
×
