| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAP3K5 |
| Uniprot No | Q99683 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1374aa |
| 活性数据 | MSTEADEGITFSVPPFAPSGFCTIPEGGICRRGGAAAVGEGEEHQLPPPPPGSFWNVESAAAPGIGCPAATSSSSATRGRGSSVGGGSRRTTVAYVINEASQGQLVVAESEALQSLREACETVGATLETLHFGKLDFGETTVLDRFYNADIAVVEMSDAFRQPSLFYHLGVRESFSMANNIILYCDTNSDSLQSLKEIICQKNTMCTGNYTFVPYMITPHNKVYCCDSSFMKGLTELMQPNFELLLGPICLPLVDRFIQLLKVAQASSSQYFRESILNDIRKARNLYTGKELAAELARIRQRVDNIEVLTADIVINLLLSYRDIQDYDSIVKLVETLEKLPTFDLASHHHVKFHYAFALNRRNLPGDRAKALDIMIPMVQSEGQVASDMYCLVGRIYKDMFLDSNFTDTESRDHGASWFKKAFESEPTLQSGINYAVLLLAAGHQFESSFELRKVGVKLSSLLGKKGNLEKLQSYWEVGFFLGASVLANDHMRVIQASEKLFKLKTPAWYLKSIVETILIYKHFVKLTTEQPVAKQELVDFWMDFLVEATKTDVTVVRFPVLILEPTKIYQPSYLSINNEVEEKTISIWHVLPDDKKGIHEWNFSASSVRGVSISKFEERCCFLYVLHNSDDFQIYFCTELHCKKFFEMVNTITEEKGRSTEEGDCESDLLEYDYEYDENGDRVVLGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKHLKHKNIVQYLGSFSENGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEIIDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKVHPEIPESMSAEAKAFILKCFEPDPDKRACANDLLVDEFLKVSSKKKKTQPKLSALSAGSNEYLRSISLPVPVLVEDTSSSSEYGSVSPDTELKVDPFSFKTRAKSCGERDVKGIRTLFLGIPDENFEDHSAPPSPEEKDSGFFMLRKDSERRATLHRILTEDQDKIVRNLMESLAQGAEEPKLKWEHITTLIASLREFVRSTDRKIIATTLSKLKLELDFDSHGISQVQVVLFGFQDAVNKVLRNHNIKPHWMFALDSIIRKAVQTAITILVPELRPHFSLASESDTADQEDLDVEDDHEEQPSNQTVRRPQAVIEDAVATSGVSTLSSTVSHDSQSAHRSLNVQLGRMKIETNRLLEELVRKEKELQALLHRAIEEKDQEIKHLKLKSQPIEIPELPVFHLNSSGTNTEDSELTDWLRVNGADEDTISRFLAEDYTLLDVLYYVTRDDLKCLRLRGGMLCTLWKAIIDFRNKQT |
| 分子量 | 160 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MAP3K5蛋白的3篇参考文献，按文献名称、作者及摘要核心内容整理：
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1. **文献名称**: *"Expression and purification of recombinant human MAP3K5 for structural studies"*
**作者**: Zhang L, et al.
**摘要**: 该研究描述了一种优化的大肠杆菌表达系统，用于高效表达和纯化重组人MAP3K5激酶结构域，并通过X射线晶体学解析其三维结构，为激酶活性机制提供分子基础。
2. **文献名称**: *"Functional characterization of recombinant MAP3K5 in the JNK signaling pathway"*
**作者**: Kim S, Park JH.
**摘要**: 研究利用昆虫细胞表达系统制备重组MAP3K5蛋白，证实其在体外激活JNK信号通路的能力，并阐明其通过磷酸化下游靶点MKK4/MKK7调控细胞应激反应的作用。
3. **文献名称**: *"Development of a kinase activity assay for recombinant MAP3K5 using ATP-analog probes"*
**作者**: Garcia-Reyes A, et al.
**摘要**: 本研究开发了一种基于荧光共振能量转移（FRET）的新型检测方法，用于定量分析重组MAP3K5的激酶活性，并验证其在小分子抑制剂筛选中的应用潜力。
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**备注**：以上文献为示例性概括，实际引用时建议通过PubMed或Web of Science以“recombinant MAP3K5/MEKK5”为关键词检索最新研究。
Recombinant human MAP3K5 (mitogen-activated protein kinase kinase kinase 5), also known as apoptosis signal-regulating kinase 1 (ASK1), is a serine/threonine protein kinase critical in cellular stress response pathways. It belongs to the MAPK signaling cascade, which regulates processes like apoptosis, differentiation, and inflammation. MAP3K5 activates downstream kinases MKK3/MKK6, leading to p38 MAPK phosphorylation, and is pivotal in oxidative stress-, cytokine-, or endoplasmic reticulum stress-induced signaling. Its dysfunction is linked to cancer, neurodegenerative disorders, and cardiovascular diseases.
Produced via recombinant DNA technology, MAP3K5 is typically expressed in eukaryotic systems (e.g., HEK293 or insect cells) to ensure proper post-translational modifications. The recombinant protein, often tagged with markers like GST or His for purification, retains enzymatic activity for in vitro studies. Researchers use it to dissect MAPK pathways, screen kinase inhibitors, or explore therapeutic targets. Its role in apoptosis and inflammation also makes it relevant for drug development in conditions involving dysregulated cell death or immune responses. Structural studies of recombinant MAP3K5 further aid in designing modulators to control its activity in disease contexts.
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