| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAP3K13 |
| Uniprot No | O43283 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-966aa |
| 活性数据 | MANFQEHLSCSSSPHLPFSESKTFNGLQDELTAMGNHPSPKLLEDQQEKGMVRTELIESVHSPVTTTVLTSVSEDSRDQFENSVLQLREHDESETAVSQGNSNTVDGESTSGTEDIKIQFSRSGSGSGGFLEGLFGCLRPVWNIIGKAYSTDYKLQQQDTWEVPFEEISELQWLGSGAQGAVFLGKFRAEEVAIKKVREQNETDIKHLRKLKHPNIIAFKGVCTQAPCYCIIMEYCAHGQLYEVLRAGRKITPRLLVDWSTGIASGMNYLHLHKIIHRDLKSPNVLVTHTDAVKISDFGTSKELSDKSTKMSFAGTVAWMAPEVIRNEPVSEKVDIWSFGVVLWELLTGEIPYKDVDSSAIIWGVGSNSLHLPVPSTCPDGFKILMKQTWQSKPRNRPSFRQTLMHLDIASADVLATPQETYFKSQAEWREEVKKHFEKIKSEGTCIHRLDEELIRRRREELRHALDIREHYERKLERANNLYMELSAIMLQLEMREKELIKREQAVEKKYPGTYKRHPVRPIIHPNAMEKLMKRKGVPHKSGMQTKRPDLLRSEGIPTTEVAPTASPLSGSPKMSTSSSKSRYRSKPRHRRGNSRGSHSDFAAILKNQPAQENSPHPTYLHQAQSQYPSLHHHNSLQQQYQQPPPAMSQSHHPRLNMHGQDIATCANNLRYFGPAAALRSPLSNHAQRQLPGSSPDLISTAMAADCWRSSEPDKGQAGPWGCCQADAYDPCLQCRPEQYGSLDIPSAEPVGRSPDLSKSPAHNPLLENAQSSEKTEENEFSGCRSESSLGTSHLGTPPALPRKTRPLQKSGDDSSEEEEGEVDSEVEFPRRQRPHRCISSCQSYSTFSSENFSVSDGEEGNTSDHSNSPDELADKLEDRLAEKLDDLLSQTPEIPIDISSHSDGLSDKECAVRRVKTQMSLGKLCVEERGYENPMQFEESDCDSSDGECSDATVRTNKHYSSATW |
| 分子量 | 134.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MAP3K13蛋白的3篇参考文献摘要示例，供参考：
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1. **文献名称**：*"Structural and functional analysis of the human MAP3K13 kinase domain"*
**作者**：Krämer, B. et al.
**摘要**：本研究解析了重组人MAP3K13激酶结构域的晶体结构，揭示了其ATP结合位点和关键调控区域。通过体外激酶实验证明其可磷酸化下游靶点MKK4.并调控JNK信号通路，提示其在细胞应激反应中的作用。
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2. **文献名称**：*"MAP3K13 modulates NF-κB activation in inflammatory responses through direct phosphorylation of IKKβ"*
**作者**：Zhang, Y. et al.
**摘要**：研究发现重组人MAP3K13蛋白通过磷酸化IKKβ激酶复合体，显著增强NF-κB信号通路的活化，促进促炎细胞因子释放。敲低MAP3K13可抑制小鼠巨噬细胞的炎症反应，表明其作为炎症治疗潜在靶点。
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3. **文献名称**：*"Role of MAP3K13 in neuronal apoptosis induced by oxidative stress"*
**作者**：Lu, Y. et al.
**摘要**：本文通过表达重组人MAP3K13蛋白发现，其在神经细胞中激活ASK1-p38通路，诱导氧化应激相关凋亡。抑制剂实验显示MAP3K13缺失可减少神经元死亡，提示其与神经退行性疾病的关联。
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注：以上文献为示例性质，实际引用时请核对具体来源及原文内容。建议通过PubMed或Web of Science以“MAP3K13”、“MEKK13”及“recombinant protein”为关键词检索最新研究。
Recombinant human MAP3K13 protein is a key component in the mitogen-activated protein kinase (MAPK) signaling pathway, which regulates cellular responses to stress, proliferation, and apoptosis. MAP3K13. also known as leucine zipper-bearing kinase (LZK), belongs to the serine/threonine protein kinase family and functions upstream of the JNK/p38 pathways. It is implicated in diverse physiological processes, including inflammation, neuronal development, and tumorigenesis. Dysregulation of MAP3K13 has been associated with cancers, neurodegenerative diseases, and metabolic disorders, highlighting its therapeutic potential.
The recombinant form of MAP3K13 is produced using expression systems like *E. coli* or mammalian cells, enabling controlled studies of its enzymatic activity, substrate interactions, and signaling mechanisms. Its recombinant production ensures high purity and consistency, critical for structural studies, inhibitor screening, and functional assays. Researchers utilize this protein to dissect MAPK cascades, explore cross-talk with other pathways, and validate drug targets. Notably, MAP3K13’s dual role in promoting cell survival or apoptosis—depending on cellular context—makes it a focus in oncology and neurobiology. Recent studies also link it to immune modulation, suggesting utility in autoimmune disease research. Overall, recombinant MAP3K13 serves as a vital tool for unraveling kinase-driven signaling networks and advancing targeted therapies.
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