| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAP3K2 |
| Uniprot No | Q9Y2U5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-619aa |
| 活性数据 | MDDQQALNSI MQDLAVLHKA SRPALSLQET RKAKSSSPKK QNDVRVKFEH RGEKRILQFP RPVKLEDLRS KAKIAFGQSM DLHYTNNELV IPLTTQDDLD KAVELLDRSI HMKSLKILLV INGSTQATNL EPLPSLEDLD NTVFGAERKK RLSIIGPTSR DRSSPPPGYI PDELHQVARN GSFTSINSEG EFIPESMDQM LDPLSLSSPE NSGSGSCPSL DSPLDGESYP KSRMPRAQSY PDNHQEFSDY DNPIFEKFGK GGTYPRRYHV SYHHQEYNDG RKTFPRARRT QGTSLRSPVS FSPTDHSLST SSGSSIFTPE YDDSRIRRRG SDIDNPTLTV MDISPPSRSP RAPTNWRLGK LLGQGAFGRV YLCYDVDTGR ELAVKQVQFD PDSPETSKEV NALECEIQLL KNLLHERIVQ YYGCLRDPQE KTLSIFMEYM PGGSIKDQLK AYGALTENVT RKYTRQILEG VHYLHSNMIV HRDIKGANIL RDSTGNVKLG DFGASKRLQT ICLSGTGMKS VTGTPYWMSP EVISGEGYGR KADIWSVACT VVEMLTEKPP WAEFEAMAAI FKIATQPTNP KLPPHVSDYT RDFLKRIFVE AKLRPSADEL LRHMFVHYH |
| 分子量 | 69,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于重组人MAP3K3(Mitogen-Activated Protein Kinase Kinase Kinase 3)蛋白的**示例参考文献**(内容基于公开研究概括，具体文献需通过数据库核实)：
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1. **文献名称**: "MAP3K3 promotes tumor progression through regulation of ERK signaling in human cancers"
**作者**: Li Y. et al.
**摘要**: 本研究揭示MAP3K3在多种癌症中过表达，通过激活ERK信号通路促进肿瘤细胞增殖和迁移。重组人MAP3K3蛋白的体外实验表明其激酶活性对肿瘤生长起关键作用。
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2. **文献名称**: "Structural basis of MAP3K3 kinase activity and its inhibition by small molecules"
**作者**: Zhang H. et al.
**摘要**: 通过X射线晶体学解析重组人MAP3K3蛋白的三维结构，揭示了其ATP结合域特征，并筛选出特异性抑制剂，为靶向治疗提供结构基础。
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3. **文献名称**: "MAP3K3 regulates immune response via JNK/p38 signaling in macrophages"
**作者**: Wang J. et al.
**摘要**: 利用重组MAP3K3蛋白研究其在巨噬细胞中的功能，发现其通过调控JNK/p38通路影响炎症因子释放，为免疫相关疾病机制提供新见解。
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4. **文献名称**: "MAP3K3 gene editing suppresses ovarian cancer metastasis in vivo"
**作者**: Chen X. et al.
**摘要**: 通过CRISPR敲除MAP3K3基因并结合重组蛋白回补实验，证明MAP3K3通过调控EMT(上皮间质转化)过程驱动卵巢癌转移。
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**注意**：以上为模拟示例，实际文献需通过PubMed、Google Scholar或Web of Science等平台检索关键词“MAP3K3”、“recombinant human MAP3K3”查询最新研究。
Recombinant human MAP3K2 (mitogen-activated protein kinase kinase kinase 2), also known as MEKK2. is a serine/threonine kinase involved in key cellular signaling pathways. As part of the MAPK/ERK cascade, it activates downstream kinases like MEK5 and MKK7. regulating processes such as cell proliferation, differentiation, and stress responses. Its structure includes an N-terminal PB1 domain for protein interactions and a C-terminal kinase domain.
MAP3K2 gained research attention due to its role in RAS-mediated oncogenesis and immune regulation. Studies link MAP3K2 mutations or overexpression to cancers, inflammatory diseases, and developmental disorders. Recombinant expression systems (e.g., E. coli, mammalian cells) enable large-scale production of purified MAP3K2 for functional studies. The protein is typically tagged (e.g., His-tag) for affinity chromatography purification.
Current applications include kinase activity assays, drug discovery for MAPK pathway inhibitors, and mechanistic studies of ERK5/JNK signaling. Researchers utilize recombinant MAP3K2 to explore its interaction with scaffold proteins like MP1 and its crosstalk with other pathways, such as NF-κB. Its dual role in pro-survival and apoptotic signals makes it a potential therapeutic target, particularly in RAS-driven cancers and autoimmune conditions. Recent CRISPR-based studies further highlight its tissue-specific regulatory functions.
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