| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAP3K15 |
| Uniprot No | Q6ZN16 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1313aa |
| 活性数据 | MESGGGNAPAGALGAASESPQCPPPPGVEGAAGPAEPDGAAEGAAGGSGE GESGGGPRRALRAVYVRSESSQGGAAGGPEAGARQCLLRACEAEGAHLTS VPFGELDFGETAVLDAFYDADVAVVDMSDVSRQPSLFYHLGVRESFDMAN NVILYHDTDADTALSLKDMVTQKNTASSGNYYFIPYIVTPCADYFCCESD AQRRASEYMQPNWDNILGPLCMPLVDRFISLLKDIHVTSCVYYKETLLND IRKAREKYQGEELAKELARIKLRMDNTEVLTSDIIINLLLSYRDIQDYDA MVKLVETLEMLPTCDLADQHNIKFHYAFALNRRNSTGDREKALQIMLQVL QSCDHPGPDMFCLCGRIYKDIFLDSDCKDDTSRDSAIEWYRKGFELQSSL YSGINLAVLLIVAGQQFETSLELRKIGVRLNSLLGRKGSLEKMNNYWDVG QFFSVSMLAHDVGKAVQAAERLFKLKPPVWYLRSLVQNLLLIRRFKKTII EHSPRQERLNFWLDIIFEATNEVTNGLRFPVLVIEPTKVYQPSYVSINNE AEERTVSLWHVSPTEMKQMHEWNFTASSIKGISLSKFDERCCFLYVHDNS DDFQIYFSTEEQCSRFFSLVKEMITNTAGSTVELEGETDGDTLEYEYDHD ANGERVVLGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIAL HKYLKHRNIVQYLGSVSENGYIKIFMEQVPGGSLSALLRSKWGPMKEPTI KFYTKQILEGLKYLHENQIVHRDIKGDNVLVNTYSGVVKISDFGTSKRLA GVNPCTETFTGTLQYMAPEIIDQGPRGYGAPADIWSLGCTIIEMATSKPP FHELGEPQAAMFKVGMFKIHPEIPEALSAEARAFILSCFEPDPHKRATTA ELLREGFLRQVNKGKKNRIAFKPSEGPRGVVLALPTQGEPMATSSSEHGS VSPDSDAQPDALFERTRAPRHHLGHLLSVPDESSALEDRGLASSPEDRDQ GLFLLRKDSERRAILYKILWEEQNQVASNLQECVAQSSEELHLSVGHIKQ IIGILRDFIRSPEHRVMATTISKLKVDLDFDSSSISQIHLVLFGFQDAVN KILRNHLIRPHWMFAMDNIIRRAVQAAVTILIPELRAHFEPTCETEGVDK DMDEAEEGYPPATGPGQEAQPHQQHLSLQLGELRQETNRLLEHLVEKERE YQNLLRQTLEQKTQELYHLQLKLKSNCITENPAGPYGQRTDKELIDWLRL QGADAKTIEKIVEEGYTLSDILNEITKEDLRYLRLRGGLLCRLWSAVSQY RRAQEASETKDKA |
| 分子量 | 180 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MAP3K15蛋白的假设性参考文献示例(仅供参考，实际文献需通过学术数据库查询)：
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1. **文献名称**：*MAP3K15 modulates β-cell apoptosis via JNK signaling in diabetes*
**作者**：Smith A, et al.
**摘要**：研究揭示了MAP3K15通过激活JNK通路促进胰腺β细胞凋亡，可能与糖尿病发病机制相关。
2. **文献名称**：*Recombinant expression and functional characterization of human MAP3K15 kinase*
**作者**：Zhang L, et al.
**摘要**：报道了人源MAP3K15蛋白的重组表达及纯化方法，并证实其在体外具有磷酸化下游底物的激酶活性。
3. **文献名称**：*A novel role of MAP3K15 in regulating ER stress response*
**作者**：Chen R, et al.
**摘要**：发现MAP3K15参与内质网应激反应调控，通过激活下游p38通路影响细胞存活。
4. **文献名称**：*Genetic variants of MAP3K15 associated with thyroid cancer progression*
**作者**：Wang Y, et al.
**摘要**：鉴定MAP3K15基因变异与甲状腺癌转移的相关性，提示其作为潜在治疗靶点。
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**注意**：以上文献为模拟示例，建议通过PubMed、Web of Science等平台以“MAP3K15 recombinant”或“MAP3K15 function”为关键词检索最新研究。
**Background of Recombinant Human MAP3K15 Protein**
The mitogen-activated protein kinase kinase kinase 15 (MAP3K15), also known as MLK4. is a member of the serine/threonine kinase family involved in regulating stress-activated signaling pathways, including the JNK and p38 MAPK cascades. Structurally, MAP3K15 contains a kinase domain at its N-terminus and a C-terminal regulatory domain with homologies to pleckstrin (PH) and PB1 domains, facilitating protein-protein interactions and cellular localization. It plays a role in mediating cellular responses to stressors, apoptosis, and differentiation, with tissue-specific expression observed in pancreatic islets, liver, and kidneys.
Research has linked MAP3K15 to metabolic and degenerative diseases. Notably, genome-wide association studies (GWAS) identified MAP3K15 as a risk gene for diabetes, potentially influencing β-cell survival and insulin secretion. Its aberrant expression has also been implicated in cancers, such as hepatocellular carcinoma, where it may promote tumor progression.
Recombinant human MAP3K15 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), retains enzymatic activity and is utilized to study kinase-substrate interactions, screen inhibitors, and unravel disease mechanisms. Its purified form enables structural analysis, aiding drug discovery efforts targeting dysregulated MAPK pathways. Current research focuses on clarifying its physiological roles and therapeutic potential in metabolic disorders and oncology.
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