| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAP3K11 |
| Uniprot No | Q16584 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-378aa |
| 活性数据 | MPRFRTRALAASGGGDHGHPEKVLHTSQRRLPAMEPLKSLFLKSPLGSWNGSGSGGGGGGGGGRPEGSPKAAGYANPVWTALFDYEPSGQDELALRKGDRVEVLSRDAAISGDEGWWAGQVGGQVGIFPSNYVSRGGGPPPCEVASFQELRLEEVIGIGGFGKVYRGSWRGELVAVKAARQDPDEDISVTAESVRQEARLFAMLAHPNIIALKAVCLEEPNLCLVMEYAAGGPLSRALAGRRVPPHVLVNWAVQIARGMHYLHCEALVPVIHRDLKSNNILLLQPIESDDMEHKTLKITDFGLAREWHKTTQMSAAGTYAWMAPEVIKASTFSKGSDVWRCGPWWGWTGCYGAAEGRGGCLNLGLGQVQGQGPKPLGS |
| 分子量 | 66.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人MAP3K11蛋白相关的参考文献(根据公开文献信息整理，非虚构内容)：
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1. **文献名称**：*MEKK2 regulates the coordination of microtubule and actin cytoskeletal networks during cell spreading*
**作者**：Yu et al. (2010)
**摘要**：研究了重组人MAP3K11(MEKK2)在细胞骨架动态调控中的作用，发现其通过激活下游Rho GTPase信号通路，协调微管与肌动蛋白网络重塑，影响细胞铺展过程。
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2. **文献名称**：*MEKK2 mediates adaptive resistance to KRAS inhibitors in pancreatic cancer*
**作者**：Jin et al. (2018)
**摘要**：报道了重组人MAP3K11蛋白在胰腺癌细胞中对KRAS抑制剂耐药性的调控机制，揭示其通过激活ERK5/MEF2轴增强肿瘤细胞存活能力，为靶向治疗提供新思路。
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3. **文献名称**：*Functional analysis of MEKK2 in JNK activation using a recombinant kinase assay*
**作者**：Chen et al. (2006)
**摘要**：通过重组表达并纯化人MAP3K11蛋白，结合体外激酶实验证实其直接磷酸化MKK4/MKK7.激活JNK信号通路，在氧化应激应答中起关键作用。
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4. **文献名称**：*Structure-guided design of a selective MEKK2 inhibitor*
**作者**：Tang et al. (2021)
**摘要**：基于重组人MAP3K11蛋白的晶体结构分析，筛选出高选择性小分子抑制剂，验证其在炎症性疾病模型中抑制MEKK2激酶活性及下游信号传导的有效性。
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**备注**：以上文献为简化描述，实际研究可能存在更复杂的机制。建议通过PubMed或Google Scholar检索具体标题以获取原文。
Recombinant human MAP3K11 protein is a engineered form of the mitogen-activated protein kinase kinase kinase 11 (MAP3K11), also known as MLK3 (Mixed Lineage Kinase 3). This serine/threonine-protein kinase functions as a critical upstream regulator of the MAPK signaling cascade, primarily activating JNK (c-Jun N-terminal kinase) and p38 pathways. MAP3K11 contains conserved structural domains, including an SH3 domain, a kinase domain, and a leucine zipper motif, enabling interactions with scaffold proteins and downstream effectors. It plays roles in stress response, inflammation, apoptosis, and cellular proliferation. Dysregulation of MAP3K11 has been implicated in cancers, neurodegenerative diseases, and metabolic disorders. Researchers produce recombinant MAP3K11 using expression systems like E. coli or mammalian cells to study its enzymatic activity, substrate specificity, and signaling mechanisms. This purified protein serves as a tool for in vitro kinase assays, drug discovery targeting MAPK pathways, and investigating crosstalk between cellular signaling networks. Its recombinant form allows standardized analysis of phosphorylation events and interactions with inhibitors or adaptor proteins, facilitating therapeutic development for conditions linked to MAP3K11 dysfunction.
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