| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAP3K3 |
| Uniprot No | Q99759 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-626aa |
| 活性数据 | MDEQEALNSI MNDLVALQMN RRHRMPGYET MKNKDTGHSN RQSDVRIKFE HNGERRIIAF SRPVKYEDVE HKVTTVFGQP LDLHYMNNEL SILLKNQDDL DKAIDILDRS SSMKSLRILL LSQDRNHNSS SPHSGVSRQV RIKASQSAGD INTIYQPPEP RSRHLSVSSQ NPGRSSPPPG YVPERQQHIA RQGSYTSINS EGEFIPETSE QCMLDPLSSA ENSLSGSCQS LDRSADSPSF RKSRMSRAQS FPDNRQEYSD RETQLYDKGV KGGTYPRRYH VSVHHKDYSD GRRTFPRIRR HQGNLFTLVP SSRSLSTNGE NMGLAVQYLD PRGRLRSADS ENALSVQERN VPTKSPSAPI NWRRGKLLGQ GAFGRVYLCY DVDTGRELAS KQVQFDPDSP ETSKEVSALE CEIQLLKNLQ HERIVQYYGC LRDRAEKTLT IFMEYMPGGS VKDQLKAYGA LTESVTRKYT RQILEGMSYL HSNMIVHRDI KGANILRDSA GNVKLGDFGA SKRLQTICMS GTGMRSVTGT PYWMSPEVIS GEGYGRKADV WSLGCTVVEM LTEKPPWAEY EAMAAIFKIA TQPTNPQLPS HISEHGRDFL RRIFVEARQR PSAEELLTHH FAQLMY |
| 分子量 | 70,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MAP3K3蛋白的3篇代表性文献摘要(虚拟示例，仅供参考)：
1. **文献名称**：*MAP3K3 regulates mitochondrial dynamics and apoptosis in cancer cells*
**作者**：Xia, H. et al.
**摘要**：研究揭示了重组人MAP3K3蛋白通过激活JNK信号通路调控线粒体分裂蛋白Drp1.促进肿瘤细胞凋亡的分子机制，为靶向癌症治疗提供新思路。
2. **文献名称**：*Structural insights into the activation mechanism of human MAP3K3 kinase*
**作者**：Chen, Y. et al.
**摘要**：利用重组人MAP3K3蛋白进行晶体结构分析，揭示了其激酶域的构象变化及ATP结合位点的调控模式，为设计特异性抑制剂奠定基础。
3. **文献名称**：*MAP3K3 promotes angiogenesis via VEGF signaling in endothelial cells*
**作者**：Wang, L. et al.
**摘要**：通过体外表达重组人MAP3K3蛋白，证实其通过ERK5/VEGF通路增强内皮细胞迁移和血管生成能力，关联其在缺血性疾病中的作用。
4. **文献名称**：*Recombinant MAP3K3 expression and its role in neurodegenerative models*
**作者**：Zhang, R. et al.
**摘要**：构建了重组人MAP3K3蛋白的HEK293稳定表达系统，发现其异常表达加剧Tau蛋白磷酸化，提示其与阿尔茨海默病的潜在关联。
(注：以上文献信息为模拟生成，实际引用需查询真实数据库如PubMed、Web of Science等。)
Recombinant human MAP3K3 (mitogen-activated protein kinase kinase kinase 3) is a genetically engineered protein widely used to study cellular signaling pathways. MAP3K3. also known as MEKK3. belongs to the serine/threonine protein kinase family and functions as a key upstream regulator of the MAPK/ERK signaling cascade. It plays critical roles in stress responses, inflammation, apoptosis, and cell proliferation by phosphorylating downstream kinases like MEK5 and MEK1/2. Structurally, MAP3K3 contains an N-terminal kinase domain and a C-terminal regulatory region. Its dysregulation has been implicated in cancers, cardiovascular diseases, and immune disorders, making it a potential therapeutic target.
Recombinant MAP3K3 is typically produced in expression systems like *E. coli* or mammalian cells, ensuring post-translational modifications for functional studies. Researchers utilize this protein to investigate signaling mechanisms, screen kinase inhibitors, and model disease-related mutations. Recent studies highlight its involvement in tumor angiogenesis, endothelial cell function, and NF-κB activation pathways. The availability of recombinant MAP3K3 accelerates drug discovery and mechanistic studies, providing tools for kinase activity assays, protein interaction analyses, and pathway validation in controlled experimental settings. Its applications extend to understanding MAP3K3's role in developmental biology and stress adaptation.
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