| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KSR2 |
| Uniprot No | Q6VAB6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-829aa |
| 活性数据 | MTDEQVCETVEKYGANREECARLNASLSCLRNVHMSGGNLSKQDWTIQWPTTETGKENNPVCPPEPTPWIRTHLSQSPRVPSKCVQHYCHTSPTPGAPVYTHVDRLTVDAYPGLCPPPPLESGHRSLPPSPRQRHAVRTPPRTPNIVTTVTPPGTPPMRKKNKLKPPGTPPPSSRKLIHLIPGFTALHRSKSHEFQLGHRVDEAHTPKAKKKSKPLNLKIHSSVGSCENIPSQQRSPLLSERSLRSFFVGHAPFLPSTPPVHTEANFSANTLSVPRWSPQIPRRDLGNSIKHRFSTKYWMSQTCTVCGKGMLFGLKCKNCKLKCHNKCTKEAPPCHLLIIHRGDPARLVRTESVPCDINNPLRKPPRYSDLHISQTLPKTNKINKDHIPVPYQPDSSSNPSSTTSSTPSSPAPPLPPSATPPSPLHPSPQCTRQQKNFNLPASHYYKYKQQFIFPDVVPVPETPTRAPQVILHPVTSNPILEGNPLLQIEVEPTSENEEVHDEAEESEDDFEEMNLSLLSARSFPRKASQTSIFLQEWDIPFEQLEIGELIGKGRFGQVYHGRWHGEVAIRLIDIERDNEDQLKAFKREVMAYRQTRHENVVLFMGACMSPPHLAIITSLCKGRTLYSVVRDAKIVLDVNKTRQIAQEIVKGMGYLHAKGILHKDLKSKNVFYDNGKVVITDFGLFSISGVLQAGRREDKLRIQNGWLCHLAPEIIRQLSPDTEEDKLPFSKHSDVFALGTIWYELHAREWPFKTQPAEAIIWQMGTGMKPNLSQIGMGKEISDILLFCWAFEQEERPTFTKLMDMLEKLPKRNRRLSHPGHFWKSAEL |
| 分子量 | 120 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KSR2蛋白的3篇文献示例(部分为模拟条目，仅供参考)：
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1. **文献名称**：*Structural and Functional Analysis of the Kinase Suppressor of Ras (KSR2) Scaffold Protein*
**作者**：Chen J, et al.
**摘要内容**：本研究通过重组表达人源KSR2蛋白，解析其三维结构，揭示了KSR2与MAPK信号通路中RAF和MEK蛋白的相互作用机制，证明其作为支架蛋白在RAS信号转导中的调控作用。
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2. **文献名称**：*Development of a Recombinant KSR2 Protein Expression System in Mammalian Cells*
**作者**：Williams R, et al.
**摘要内容**：文章报道了一种在HEK293细胞中高效表达重组人KSR2蛋白的方法，并验证了其与EGFR信号通路的关联性，为研究KSR2在癌症中的功能提供了工具。
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3. **文献名称**：*KSR2 Regulates Cellular Energy Metabolism via AMPK Signaling*
**作者**：Liu Y, et al.
**摘要内容**：作者利用纯化的重组KSR2蛋白，结合体外实验发现KSR2通过调控AMPK活性影响细胞代谢平衡，暗示其在肥胖和糖尿病中的潜在作用。
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**注**：以上文献信息为示例，实际研究中请通过学术数据库(如PubMed、Web of Science)检索最新文献，以确保准确性。建议使用关键词 "recombinant human KSR2" 或 "KSR2 protein expression" 进行搜索。
Kinase Suppressor of Ras 2 (KSR2) is a scaffolding protein that plays a critical role in the RAS/mitogen-activated protein kinase (MAPK) signaling pathway, which regulates cell proliferation, differentiation, and survival. As a pseudokinase, KSR2 lacks catalytic activity but facilitates signal transduction by assembling components of the RAF-MEK-ERK cascade, enhancing the efficiency and specificity of kinase interactions. It is particularly important in metabolic regulation, with studies linking KSR2 mutations to obesity, insulin resistance, and neurological disorders. Recombinant human KSR2 protein is engineered for research applications, typically produced in heterologous systems like *E. coli* or mammalian cells, followed by purification to study its structure-function relationships or screen therapeutic agents. Its role in cancer—modulating RAS-driven oncogenesis—has spurred interest in KSR2-targeted therapies. The recombinant protein enables biochemical assays, *in vitro* phosphorylation studies, and exploration of KSR2's interplay with BRAF, MEK, or other signaling partners. Advances in structural biology, including cryo-EM, have clarified how KSR2 orchestrates RAF dimerization and activation, providing a foundation for developing allosteric inhibitors or stabilizers. Research on recombinant KSR2 continues to illuminate its dual regulatory roles in physiology and disease.
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