| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRTHA4 |
| Uniprot No | O76011 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-436aa |
| 活性数据 | MLYAKPPPTINGIKGLQRKERLKPAHIHLQQLTCFSITCSSTMSYSCCLPSLGCRTSCSSRPCVPPSCHGYTLPGACNIPANVSNCNWFCEGSFNGSEKETMQFLNDRLASYLEKVRQLERDNAELEKLIQERSQQQEPLLCPSYQSYFKTIEELQQKILCAKAENARLVVNIDNAKLASDDFRSKYQTEQSLRLLVESDINSIRRILDELTLCKSDLESQVESLREELICLKKNHEEEVNTLRSQLGDRLNVEVDTAPTVDLNQVLNETRSQYEALVETNRREVEQWFATQTEELNKQVVSSSEQLQSCQAEIIELRRTVNALEIELQAQHNLRDSLENTLTESEAHYSSQLSQVQSLITNVESQLAEIRCDLERQNQEYQVLLDVRARLECEINTYRSLLESEDCKLPCNPCATTNASGNSCGPCGTSQKGCCN |
| 分子量 | 75.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KRTHA4蛋白的参考文献示例(虚构内容，仅供格式参考)：
1. **标题**：Expression and characterization of recombinant human KRTHA4 in *E. coli*
**作者**：Smith A, et al.
**摘要**：研究利用大肠杆菌系统成功表达了重组人KRTHA4蛋白，并通过色谱法纯化，验证了其与天然蛋白相似的免疫反应性和热稳定性。
2. **标题**：Structural insights into KRTHA4 protein by X-ray crystallography
**作者**：Chen L, et al.
**摘要**：通过X射线晶体学解析了KRTHA4的三维结构，发现其α-螺旋结构域对毛发角质纤维的力学性能有重要影响，为相关遗传疾病研究提供依据。
3. **标题**：KRTHA4 functional analysis in hair follicle development using transgenic mice
**作者**：Yamamoto H, et al.
**摘要**：构建KRTHA4敲除小鼠模型，发现该蛋白缺失导致毛干结构异常;体外实验证实重组KRTHA4可部分修复缺陷表型。
4. **标题**：Proteomic screening of KRTHA4 interaction partners in keratinocytes
**作者**：Garcia R, et al.
**摘要**：通过质谱分析鉴定出KRTHA4与角蛋白KRT85及交联酶TGM3的相互作用，揭示其在毛发角质化细胞中的网络调控机制。
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注：KRTHA4(*Keratin, Hair, Acidic*)属于毛发特异性角蛋白家族，实际研究文献较少。如需真实文献，建议通过PubMed或Google Scholar检索“KRTHA4”或“hair keratin”相关关键词，或验证基因命名(部分文献可能使用旧称如KRT或HHa命名系统)。
Recombinant human KRTHA4 protein is a engineered form of the keratin-associated protein 4 (KRTAP4-4) encoded by the *KRTHA4* gene, belonging to the keratin-associated protein (KAP) family. Keratins are structural components of hair and nails, classified into type I (acidic) and type II (basic) proteins. KAPs, including KRTHA4. are critical for forming disulfide bonds with keratins to stabilize the hair shaft's ultra-structure. KRTHA4 is specifically expressed in the hair cortex and contributes to the mechanical strength, elasticity, and chemical resistance of hair fibers.
Research on recombinant KRTHA4 stems from interests in hair biology and disorders like congenital hair shaft abnormalities or alopecia. Produced via recombinant DNA technology in bacterial or mammalian systems, the protein retains functional domains for keratin binding and disulfide crosslinking. It serves as a tool to study hair formation mechanisms, keratin network interactions, and the impact of genetic mutations on hair integrity. Additionally, it has potential applications in cosmetic science for evaluating hair-care products or developing treatments for hair damage. Studies also explore its role beyond hair, including epithelial cell stress responses and possible associations with certain cancers, though these areas remain exploratory. Overall, KRTHA4 recombinant protein bridges basic research and translational applications in dermatology and material science.
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