| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRTCAP2 |
| Uniprot No | Q8N6L1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-162aa |
| 活性数据 | MRIANRTRFSSPFLARGAGWTHGRGMMVVGTGTSLALSSLLSLLLFAGMQMYSRQLASTEWLTIQGGLLGSGLFVFSLTAFNNLENLVFGKGFQAKIFPEILLCLLLALFASGLIHRVCVTTCFIFSMVGLYYINKISSTLYQAAAPVLTPAKVTGKSKKRN |
| 分子量 | 44 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人KRTCAP2蛋白的模拟参考文献(注：文献信息为示例性模拟，非真实存在)：
---
1. **文献名称**: *Structural insights into recombinant human KRTCAP2 and its role in potassium channel regulation*
**作者**: Smith, J., et al. (2018)
**摘要**: 本研究解析了重组人KRTCAP2蛋白的晶体结构，发现其通过形成四聚体与钾通道亚基结合，可能参与维持细胞膜电位的稳定性，为钾通道功能调控机制提供了新依据。
2. **文献名称**: *KRTCAP2 promotes cancer cell migration via recombinant protein interaction studies*
**作者**: Zhang, L., & Lee, Y. (2020)
**摘要**: 通过体外表达重组人KRTCAP2蛋白，证实其过表达能增强乳腺癌细胞的迁移能力，可能与调控MAPK/ERK信号通路及细胞骨架重构相关。
3. **文献名称**: *Proteomic analysis of KRTCAP2-binding partners using recombinant protein pulldown assays*
**作者**: Chen, R., et al. (2021)
**摘要**: 利用重组KRTCAP2蛋白进行亲和纯化-质谱分析，鉴定出多个新型互作蛋白(如KCNQ家族成员)，提示其在离子通道复合物组装中的潜在作用。
---
**备注**：以上文献为模拟生成，实际研究中建议通过权威数据库(如PubMed、Google Scholar)以“KRTCAP2”、“recombinant KRTCAP2”等关键词检索真实文献。
Recombinant human KRTCAP2 (Keratinocyte-Associated Protein 2) is a engineered protein derived from the KRTCAP2 gene, which encodes a small, cysteine-rich protein primarily expressed in epithelial tissues. KRTCAP2 is implicated in regulating cytoskeletal dynamics by interacting with keratin intermediate filaments, playing a role in maintaining cellular structure, adhesion, and mechanical integrity. Its involvement in epithelial differentiation and stress response suggests potential contributions to tissue homeostasis and wound repair. Structurally, it contains conserved motifs that mediate protein-protein interactions, particularly with keratins like KRT18.
The recombinant form is typically produced in bacterial (e.g., E. coli) or mammalian expression systems to enable functional studies. Purified recombinant KRTCAP2 serves as a valuable tool for investigating epithelial cell biology, including roles in tumor progression (notably in carcinomas) and inflammatory skin disorders. Emerging research explores its interaction networks in cellular signaling pathways and potential as a biomarker. Challenges in studying native KRTCAP2. such as low endogenous expression and solubility, are mitigated by recombinant overexpression systems. Current applications extend to structural analysis, antibody development, and mechanistic studies of keratin-related pathologies. Its conserved nature across vertebrates underscores its fundamental role in epithelial physiology.
×
