| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRTAP9-8 |
| Uniprot No | Q9BYQ0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-159aa |
| 活性数据 | MTHCCSPCCQPTCCRTTCWKPTTVTTCSSTPCCQPSCCVSSCCQPCCRPTCCQNTCCQPICVTSCCQPSCCSTPCCQPTCCGQTSCGSSCGQSSSCAPVYCRRTCYHPTTVCLPGCLNQSCGSSCCQPCCRPACCETTCCRTTCFQPTCVYSCCQPSCC |
| 分子量 | 43.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为基于研究领域背景生成的示例参考文献(注意：所列内容为模拟示例，实际文献请通过学术数据库检索)：
1. **《重组人KRTAP9-8蛋白在大肠杆菌中的高效表达与纯化》**
作者：Zhang L. et al.
摘要：研究实现了人KRTAP9-8蛋白在原核系统中的可溶性表达，通过优化密码子和纯化流程获得高纯度蛋白，为其结构解析提供实验基础。
2. **《KRTAP9-8蛋白在毛发角蛋白纤维形成中的作用机制》**
作者：Smith J.R. & Chen H.
摘要：通过基因敲除模型，发现KRTAP9-8缺失导致角蛋白交联异常，表明其对毛囊细胞力学稳定性具有调控作用。
3. **《人KRTAP9-8蛋白的结构预测及与角蛋白相互作用分析》**
作者：Wang Y. et al.
摘要：利用分子建模和体外结合实验，揭示了KRTAP9-8蛋白中保守的螺旋区域对结合角蛋白K81的关键功能位点。
4. **《重组KRTAP9-8在皮肤屏障修复中的潜在应用》**
作者：Li M. et al.
摘要：体外实验表明，重组KRTAP9-8可促进表皮细胞迁移和分化，提示其在皮肤损伤修复中的治疗潜力。
建议通过 **PubMed/Google Scholar** 检索关键词“KRTAP9-8 recombinant human”或“KRTAP9-8 protein function”获取真实文献。
Recombinant human KRTAP9-8 protein is a genetically engineered variant of the keratin-associated protein 9-8 (KRTAP9-8), a member of the keratin-associated protein (KAP) family. These proteins are critical components of hair and skin structures, contributing to the mechanical strength, flexibility, and integrity of keratinized tissues. KRTAP9-8. located within the human hair keratin-associated protein gene cluster on chromosome 21q22.1. is specifically expressed in the hair follicle cortex and plays a role in disulfide bond crosslinking during hair shaft formation. Its function is closely linked to hair texture, density, and resistance to environmental stress.
Recombinant KRTAP9-8 is produced using heterologous expression systems (e.g., E. coli, yeast, or mammalian cells) to enable controlled study of its biochemical properties and interactions. Researchers utilize this protein to investigate hair biology, genetic disorders affecting keratinization, and potential therapeutic applications in conditions like alopecia or brittle hair syndromes. Its production often involves codon optimization and purification tags to enhance solubility and yield due to the native protein's structural complexity. Current studies focus on deciphering its role in hair fiber assembly and exploring its utility in biomaterials or cosmetic formulations aimed at improving hair health.
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