| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRTHB1 |
| Uniprot No | Q14533 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-505aa |
| 活性数据 | MTCGSGFGGR AFSCISACGP RPGRCCITAA PYRGISCYRG LTGGFGSHSV CGGFRAGSCG RSFGYRSGGV CGPSPPCITT VSVNESLLTP LNLEIDPNAQ CVKQEEKEQI KSLNSRFAAF IDKVRFLEQQ NKLLETKLQF YQNRECCQSN LEPLFEGYIE TLRREAECVE ADSGRLASEL NHVQEVLEGY KKKYEEEVSL RATAENEFVA LKKDVDCAYL RKSDLEANVE ALIQEIDFLR RLYEEEILIL QSHISDTSVV VKLDNSRDLN MDCIIAEIKA QYDDIVTRSR AEAESWYRSK CEEMKATVIR HGETLRRTKE EINELNRMIQ RLTAEVENAK CQNSKLEAAV AQSEQQGEAA LSDARCKLAE LEGALQKAKQ DMACLIREYQ EVMNSKLGLD IEIATYRRLL EGEEQRLCEG IGAVNVCVSS SRGGVVCGDL CVSGSRPVTG SVCSAPCNGN VAVSTGLCAP CGQLNTTCGG GSCGVGSCGI SSLGVGSCGS SCRKC |
| 分子量 | 54.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KRTHB1蛋白的假想参考文献示例，格式符合要求：
1. **文献名称**：*Expression and purification of recombinant human KRTHB1 in Escherichia coli*
**作者**：Smith, J.R., et al.
**摘要**：研究报道了在大肠杆菌中高效表达重组人KRTHB1蛋白的优化方法，通过His标签亲和层析纯化，并验证其与天然毛发角蛋白的结构相似性。
2. **文献名称**：*Structural characterization of KRTHB1 reveals critical disulfide bonds in hair keratin assembly*
**作者**：Li, X., & Tanaka, K.
**摘要**：利用X射线晶体学解析了重组KRTHB1的三维结构，揭示了二硫键对其在毛发纤维中形成聚合结构的关键作用。
3. **文献名称**：*KRTHB1 mutations alter protein stability in hereditary hair disorders*
**作者**：Garcia, M., et al.
**摘要**：通过构建重组KRTHB1突变体，发现某些遗传性脱发相关突变导致蛋白稳定性下降，为病理机制提供分子依据。
4. **文献名称**：*Recombinant KRTHB1 as a biomaterial for hair follicle regeneration*
**作者**：Chen, W., et al.
**摘要**：探索重组KRTHB1蛋白在体外促进毛囊干细胞分化的潜力，提出其在组织工程中的应用前景。
(注：以上文献为示例性内容，实际文献需通过学术数据库检索。)
Keratin-associated protein 1 (KRTHB1), also known as hair keratin type II member 1. is a structural protein predominantly expressed in hair follicles and nails. Belonging to the type II keratin family, it plays a critical role in forming intermediate filaments that provide mechanical resilience to hair shafts and epidermal appendages. KRTHB1 interacts with type I keratins to assemble into heteropolymers, establishing a fibrous network essential for hair texture, strength, and elasticity. Mutations in the KRT81 gene encoding KRTHB1 are linked to hereditary hair disorders, such as monilethrix and pseudomonilethrix, characterized by brittle hair, alopecia, and structural shaft abnormalities.
Recombinant human KRTHB1 protein is produced using heterologous expression systems (e.g., E. coli, mammalian cells) for functional studies. Its production enables researchers to investigate keratin polymerization dynamics, protein-protein interactions, and molecular mechanisms underlying hair fragility. It also serves as a tool for screening therapeutic compounds targeting keratin-related pathologies. Applications extend to cosmetic science for evaluating hair care products and understanding environmental stress effects on keratin integrity. Despite challenges in preserving post-translational modifications in prokaryotic systems, recombinant KRTHB1 remains vital for advancing hair biology research and translational efforts in dermatology and genetic medicine.
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