| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRTHA3B |
| Uniprot No | Q14525 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-404aa |
| 活性数据 | MPYNFCLPSLSCRTSCSSRPCVPPSCHGYTLPGACNIPANVSNCNWFCEGSFNGSEKETMQFLNDRLASYLEKVRQLERDNAELENLIRERSQQQEPLLCPSYQSYFKTIEELQQKILCSKSENARLVVQIDNAKLAADDFRTKYQTEQSLRQLVESDINSLRRILDELTLCRSDLEAQMESLKEELLSLKQNHEQEVNTLRCQLGDRLNVEVDAAPAVDLNQVLNETRNQYEALVETNRREVEQWFATQTEELNKQVVSSSEQLQSYQAEIIELRRTVNALEIELQAQHNLRYSLENTLTESEARYSSQLSQVQSLITNVESQLAEIRSDLERQNQEYQVLLDVRARLECEINTYRSLLESEDCKLPSNPCATTNACEKPIGSCVTNPCGPRSRCGPCNTFGY |
| 分子量 | 70.18 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KRTHA3B蛋白的相关文献示例(内容为模拟整理，仅供参考)：
1. **文献名称**: *Structural characterization of human keratin-associated protein KRTHA3B*
**作者**: Smith J, et al.
**摘要**: 研究通过大肠杆菌重组表达KRTHA3B蛋白，利用X射线晶体学解析其三维结构，揭示了其在毛发角蛋白组装中的潜在相互作用位点。
2. **文献名称**: *Expression and functional analysis of KRTHA3B in hair follicle development*
**作者**: Lee H, et al.
**摘要**: 利用哺乳动物细胞系统(HEK293)成功表达重组KRTHA3B蛋白，体外实验表明其参与调控毛囊细胞分化，可能与遗传性毛发疾病相关。
3. **文献名称**: *KRTHA3B mutations in monilethrix: Insights from recombinant protein studies*
**作者**: García F, et al.
**摘要**: 构建KRTHA3B突变体重组蛋白模型，发现特定突变(如p.Arg117Cys)导致蛋白稳定性下降，为单珠状发(monilethrix)的分子机制提供证据。
4. **文献名称**: *Proteomic profiling of human keratins: Recombinant KRTHA3B as a potential biomarker*
**作者**: Wang X, et al.
**摘要**: 开发重组KRTHA3B抗体并通过质谱分析验证其在头皮组织中的表达特异性，提示其可作为毛发相关疾病的潜在生物标志物。
**注意**：以上文献为示例性内容，实际引用需通过学术数据库(如PubMed、Web of Science)验证真实性及获取完整信息。
Recombinant human KRTHA3B protein is a engineered form of the keratin-associated protein 3B (KRTAP3B), a member of the human hair keratin-associated protein (KAP) family. Keratins are structural components of hair and nails, categorized into two types: acidic Type I and basic Type II. KRTHA3B, as a Type I hair keratin, is rich in cysteine residues, contributing to disulfide bond formation critical for hair shaft integrity. It is predominantly expressed in the hair follicle cortex during hair fiber formation, playing a role in maintaining mechanical strength, elasticity, and structural organization of hair.
Research on recombinant KRTHA3B focuses on elucidating its interactions with other keratins and KAPs, as well as its role in hair disorders like monilethrix or hereditary hair defects. The recombinant protein is typically produced in bacterial or mammalian expression systems, enabling studies on its biochemical properties, post-translational modifications, and structure-function relationships. Its applications extend to developing diagnostic tools for genetic hair diseases and exploring therapeutic strategies, including gene editing or protein-based treatments. Additionally, it serves as a model for understanding epithelial cytoskeleton dynamics and keratinopathies. Studies using recombinant KRTHA3B have also advanced insights into evolutionary adaptations of hair proteins across species.
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