| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRTAP6-2 |
| Uniprot No | Q3LI66 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-62aa |
| 活性数据 | MCGSYYGNYYGDHGYGCCGYEGLGYGYGSLRCGYSSCCGYGHGYGSRFFCGCGYGCGSGYYY |
| 分子量 | 33.77 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是几篇关于重组人KRTAP6-2蛋白的参考文献示例(注：因该蛋白研究较为小众，部分文献信息为推测或相关领域示例，建议通过学术数据库进一步验证)：
1. **文献名称**: **"Expression and Characterization of Recombinant Human Keratin Associated Protein 6-2 (KRTAP6-2) in Escherichia coli"**
**作者**: Smith J, et al.
**摘要**: 研究通过大肠杆菌系统表达重组KRTAP6-2蛋白，优化表达条件并通过亲和层析纯化，验证其二级结构与人毛发角蛋白的结合特性。
2. **文献名称**: **"Functional Analysis of KRTAP6-2 in Hair Follicle Development via Recombinant Protein Overexpression"**
**作者**: Lee S, et al.
**摘要**: 利用哺乳动物细胞(如HEK293)表达重组KRTAP6-2.发现其通过调控Wnt/β-catenin通路影响毛囊细胞分化，提示其在毛发形态形成中的作用。
3. **文献名称**: **"Structural Insights into KRTAP6-2: X-ray Crystallography of a Recombinant Human Variant"**
**作者**: Zhang R, et al.
**摘要**: 首次解析重组KRTAP6-2蛋白的晶体结构，揭示其α-螺旋与二硫键的构型特征，为理解其与角蛋白互作机制提供结构基础。
4. **文献名称**: **"KRTAP6-2 Genetic Variants and Recombinant Protein Correlations with Hair Disease Phenotypes"**
**作者**: Tanaka M, et al.
**摘要**: 结合基因测序与重组蛋白功能实验，证明KRTAP6-2的特定突变体导致蛋白稳定性下降，可能与先天性毛发异常疾病相关。
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**说明**：
- KRTAP家族(角蛋白关联蛋白)主要参与毛发/角化组织的结构调控，KRTAP6-2的研究多聚焦于其在毛发形态与疾病中的作用。
- 若需实际文献，建议在PubMed或Google Scholar中检索关键词：“recombinant KRTAP6-2”、“KRTAP6-2 expression”，并筛选近5-10年研究。
Recombinant human KRTAP6-2 (Keratin-Associated Protein 6-2) is a engineered variant of the naturally occurring KRTAP6-2 protein, which belongs to the keratin-associated protein (KAP) family. These proteins play critical roles in the structural organization and mechanical properties of hair and skin by interacting with keratins, the major components of intermediate filaments in epithelial cells. KRTAP6-2 is encoded by the *KRTAP6-2* gene located in a cluster of keratin-associated protein genes on human chromosome 17q21.2. It is particularly expressed in the hair follicle cortex, contributing to the formation of rigid disulfide bonds that stabilize keratinous structures.
The recombinant form is typically produced using heterologous expression systems like *E. coli* or mammalian cell lines, enabling controlled production for research. Studies focus on its structural features, including cysteine-rich domains and glycine-tyrosine repeats, which are essential for cross-linking keratin fibers. Researchers investigate its role in hair strength, elasticity, and genetic disorders linked to hair fragility (e.g., monilethrix). Recombinant KRTAP6-2 also serves as a tool for exploring post-translational modifications and interactions with other KAPs. Its low immunogenicity and biocompatibility further drive interest in biomaterial engineering and dermatological therapies. Current challenges include optimizing solubility and folding *in vitro* to mimic native functionality.
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