| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRTAP8-1 |
| Uniprot No | Q8IUC2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-63aa |
| 活性数据 | MLCDNFPGAVFPGCYWGSYGYPLGYSVGCGYGSTYSPVGYGFGYGYNGCGAFGYRRYSPFALY |
| 分子量 | 7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于知识推测生成的模拟参考文献示例(非真实文献，建议通过学术数据库查询最新研究)：
1. **《重组人KRTAP8-1蛋白的表达及其结构特征》**
*作者：Wang X, et al.*
摘要：研究通过大肠杆菌系统成功表达重组人KRTAP8-1蛋白，并利用圆二色谱分析其二级结构，发现其富含α-螺旋结构，可能与角蛋白纤维组装相关。
2. **《KRTAP8-1基因多态性与毛发疾病关联研究》**
*作者：Chen L, et al.*
摘要：首次发现KRTAP8-1基因SNP变异与遗传性毛发脆弱症相关，体外重组蛋白功能实验表明突变体影响角蛋白网络稳定性。
3. **《KRTAP8-1在表皮干细胞分化中的调控作用》**
*作者：Kimura R, et al.*
摘要：通过CRISPR/Cas9敲低KRTAP8-1基因，发现其通过Wnt/β-catenin通路调控表皮干细胞向终末分化角质细胞转化，重组蛋白可部分挽救分化表型。
4. **《重组KRTAP8-1蛋白的纯化与抗体开发》**
*作者：Zhang Y, et al.*
摘要：优化了真核细胞表达系统的重组蛋白纯化方案，并制备了高特异性单克隆抗体，为后续临床样本检测奠定基础。
**建议**：使用PubMed、Web of Science或Google Scholar，以“KRTAP8-1 recombinant protein”或“KRTAP8-1 functional study”为关键词检索最新文献，重点关注涉及蛋白重组表达、功能机制或疾病关联的研究。
**Background of Recombinant Human KRTAP8-1 Protein**
Keratin-associated protein 8-1 (KRTAP8-1) is a member of the keratin-associated protein (KAP) family, which plays critical roles in forming and stabilizing hair keratin intermediate filaments. These proteins are essential for the structural integrity and mechanical properties of hair fibers. KRTAP8-1 is specifically expressed in the hair follicle cortex, where it contributes to disulfide bond-mediated crosslinking during hair shaft formation, influencing hair texture, strength, and elasticity.
The recombinant form of human KRTAP8-1 is produced using biotechnological systems (e.g., *E. coli* or mammalian cell cultures) to enable large-scale, pure protein yields for functional studies. Its recombinant production facilitates research into hair biology, genetic hair disorders (e.g., monilethrix or trichothiodystrophy), and interactions between KAPs and keratins. Additionally, KRTAP8-1 has potential applications in cosmetic and biomedical industries, particularly in developing treatments for hair damage or loss. Studies on polymorphisms or mutations in the *KRTAP8-1* gene also provide insights into evolutionary adaptations of hair traits across populations. Overall, recombinant KRTAP8-1 serves as a valuable tool for decoding hair morphogenesis and related pathologies.
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