| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRTAP5-6 |
| Uniprot No | Q6L8G9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-129aa |
| 活性数据 | MGCCGCSGGC GSGCGGCGSG CGGCGSSCCV PICCCKPVCC CVPACSCTSC GSCGGSKGCC GSCGGSKGGC GSCGGSKGGC GSCGCSQCSC CKPCYCSSGC GSSCCQSSCC KPCCSQASCC VPICCQCKI |
| 分子量 | 11.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为基于现有文献推测或示例的重组人KRTAP5-6蛋白相关参考文献(若实际研究较少，部分信息可能为示例性内容，建议通过学术数据库进一步核实)：
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1. **文献名称**: "Expression and Characterization of Recombinant Human KRTAP5-6 Protein in Mammalian Cells"
**作者**: Smith A, et al.
**摘要**: 该研究构建了人KRTAP5-6基因的真核表达系统，通过哺乳动物细胞(如HEK293)成功表达并纯化重组蛋白。实验表明，KRTAP5-6可能与毛纤维的硬角化过程相关，并影响毛发力学性能。
2. **文献名称**: "Genetic Variation in KRTAP5-6 and Its Association with Hair Follicle Traits in Human Populations"
**作者**: Li Y, et al.
**摘要**: 通过群体遗传学分析，发现KRTAP5-6基因多态性与人类毛发的直径和卷曲度显著相关。重组蛋白实验表明，特定突变体可能改变角蛋白网络的稳定性。
3. **文献名称**: "Structural Insights into KRTAP5-6 Interaction with Keratin Proteins by Recombinant Co-Expression Studies"
**作者**: Tanaka K, et al.
**摘要**: 利用重组共表达技术研究KRTAP5-6与角蛋白(如KRT82)的相互作用，发现其通过硫醇键参与毛发角蛋白基质的组装，并解析了关键结构域的功能。
4. **文献名称**: "Functional Analysis of Recombinant KRTAP5-6 in Skin Barrier Formation"
**作者**: Wang X, et al.
**摘要**: 重组KRTAP5-6蛋白在皮肤表皮细胞中的功能研究表明，其表达水平影响表皮屏障的完整性，并与特应性皮炎等疾病中角蛋白异常相关。
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**注**：上述文献为模拟示例，实际研究可能有限。建议通过**PubMed**或**Google Scholar**以关键词“recombinant KRTAP5-6”或“KRTAP5-6 protein expression”检索最新文献。
The human KRTAP5-6 protein, encoded by the *KRTAP5-6* gene, belongs to the keratin-associated protein (KAP) family, which plays critical roles in the structural integrity and mechanical properties of hair and skin. KAPs are cysteine-rich, matrix-forming proteins that interact with keratin intermediate filaments to establish resilient tissue architectures. Specifically, KRTAP5-6 is a member of the high sulfur-containing KAP group, contributing to hair shaft strength, elasticity, and cross-linking through disulfide bonds. Its expression is primarily restricted to hair follicle keratinocytes during hair differentiation.
Recombinant human KRTAP5-6 is synthesized using biotechnological platforms (e.g., bacterial or mammalian expression systems) to produce purified, functional protein for research and industrial applications. Studying recombinant KRTAP5-6 aids in elucidating molecular mechanisms underlying hair disorders, genetic variations linked to hair fragility, and potential therapeutic targets. It also serves as a model for biomaterial development due to its structural properties. Challenges in production include optimizing solubility and post-translational modifications to mimic native conformations. Current research focuses on its interaction networks with keratins and applications in cosmetic science or tissue engineering. Further exploration of KRTAP5-6 may advance personalized hair-care solutions and regenerative medicine.
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