| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRTAP4-12 |
| Uniprot No | Q9BQ66 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-201aa |
| 活性数据 | MVNSCCGSVCSDQGCGLENCCRPSCCQTTCCRTTCCRPSCCVSSCCRPQCCQSVCCQPTCCRPSCCQTTCCRTTCCRPSCCVSSCCRPQCCQSVCCQPTCCRPSCCQTTCCRTTCCRPSCCVSSCCRPQCCQSVCCQPTCCRPSCCISSSCCPSCCESSCCRPCCCLRPVCGRVSCHTTCYRPTCVISTCPRPLCCASSCC |
| 分子量 | 48.51 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于KRTAP蛋白家族研究的常见方向，推测的3篇关于重组人KRTAP4-12蛋白的假设性参考文献(内容为模拟概括，供参考)：
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1. **文献名称**：*Expression and Characterization of Recombinant Human KRTAP4-12 in Escherichia coli*
**作者**：Zhang L, et al.
**摘要**：本研究成功构建了人KRTAP4-12基因的原核表达系统，通过IPTG诱导在大肠杆菌中高效表达重组蛋白。经His标签纯化后，利用圆二色谱分析证实其具有β-螺旋结构，可能与毛发角蛋白的机械强度有关。
2. **文献名称**：*Genetic Variation in KRTAP4-12 Influences Hair Fiber Ultrastructure in Human Populations*
**作者**：Smith J, et al.
**摘要**：通过对不同人群的KRTAP4-12基因测序，发现其编码区SNP rs123456与毛发直径和弯曲度显著相关。重组蛋白体外实验表明，该变异导致蛋白二硫键形成能力改变，可能影响毛发纤维的稳定性。
3. **文献名称**：*KRTAP4-12 Interaction with Trichohyalin in Keratinocyte Differentiation*
**作者**：Tanaka K, et al.
**摘要**：利用免疫共沉淀和重组KRTAP4-12蛋白验证，发现其与毛囊分化标志物Trichohyalin直接结合，并在小鼠模型中证实该互作对毛囊角质化过程具有调控作用，提示其在脱发疾病中的潜在功能。
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**注意**：上述文献为模拟生成，实际研究中KRTAP4-12的具体功能可能仍需实验验证。建议通过 **PubMed** 或 **Google Scholar** 以关键词 "KRTAP4-12 recombinant" 或 "KRTAP4-12 protein function" 检索最新文献。如需真实文献，可进一步提供具体研究方向，辅助精准查询。
**Background of Recombinant Human KRTAP4-12 Protein**
The Keratin-associated protein 4-12 (KRTAP4-12) belongs to the keratin-associated protein (KAP) family, which plays a crucial role in forming the structural framework of hair and skin. KAPs interact with keratin intermediate filaments to stabilize tissue integrity, contributing to the mechanical strength and elasticity of epithelial structures. KRTAP4-12. encoded by the *KRTAP4-12* gene, is a cysteine- and glycine-rich protein predominantly expressed in hair follicles. Its sequence features repetitive motifs that facilitate disulfide bond formation, critical for cross-linking keratins and ensuring cohesive matrix assembly.
Recombinant human KRTAP4-12 is produced using biotechnological systems like *E. coli* or mammalian cell cultures, enabling controlled synthesis for research and applications. Studies on this protein are limited but suggest its involvement in hair shaft formation and cuticle resilience. Dysregulation of KRTAPs has been linked to hair disorders, positioning KRTAP4-12 as a potential biomarker or therapeutic target.
Current research focuses on characterizing its physicochemical properties, post-translational modifications, and interactions with keratins. Applications span cosmetic science (e.g., hair repair products), bio-material engineering, and understanding genetic hair pathologies. Further exploration of KRTAP4-12 could advance precision dermatology and regenerative medicine strategies.
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