| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRTAP5-11 |
| Uniprot No | Q6L8G4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-156aa |
| 活性数据 | MGCCGCSGGCGSGCGGCGSGSGGCGSGCGGCGSSCCVPICCCKPVCCCVPACSCSSCGSCGGSKGGCGSCGSSKGGCGSCGCSQSNCCKPCCSSSGCGSFCCQSSCSKPCCCQSSCCQSSCCKPCCCQSSCCQSSCFKPCCCQSSCCVPVCCQCKI |
| 分子量 | 43.56 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KRTAP5-11蛋白的示例参考文献(注：以下内容为模拟生成的示例，建议通过学术数据库检索真实文献)：
1. **《Expression and characterization of recombinant human KRTAP5-11 in Escherichia coli》**
- **作者**: Li X., Zhang Y., et al.
- **摘要**: 本研究成功构建了KRTAP5-11的原核表达系统，通过大肠杆菌重组表达并纯化出可溶性蛋白，验证了其与角蛋白的相互作用能力，为后续功能研究奠定基础。
2. **《Structural analysis of KRTAP5-11 reveals its role in hair shaft rigidity》**
- **作者**: Wang H., Suzuki T., et al.
- **摘要**: 利用X射线晶体学解析了重组人KRTAP5-11的三维结构，发现其通过α螺旋结构域增强毛干角蛋白交联，提出其在毛发机械强度中的潜在功能。
3. **《Genetic variants of KRTAP5-11 associated with alopecia in human populations》**
- **作者**: Chen J., Roberts G., et al.
- **摘要**: 通过全基因组关联分析发现，KRTAP5-11基因的突变与遗传性脱发相关，体外实验表明重组蛋白的异常表达影响角质细胞分化，提示其在毛发疾病中的重要性。
建议通过PubMed、Google Scholar等平台检索实际文献，结合关键词如“KRTAP5-11 recombinant protein”、“keratin-associated protein function”进一步获取资料。
**Background of Recombinant Human KRTAP5-11 Protein**
The keratin-associated protein 5-11 (KRTAP5-11) is a member of the keratin-associated protein (KAP) family, which plays a crucial role in hair shaft formation and structural integrity. These proteins interact with keratins to stabilize intermediate filaments, contributing to the mechanical strength and elasticity of hair. KRTAP5-11. encoded by the *KRTAP5-11* gene located on human chromosome 11. is enriched in cysteine residues, a hallmark of KAPs that facilitates disulfide bond formation critical for hair matrix stability.
Recombinant human KRTAP5-11 protein is engineered via heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its biochemical properties and functions. Its production enables investigations into hair biology, including disorders like alopecia or hair fragility, and supports research on genetic variations (polymorphisms) linked to hair texture or disease susceptibility. Additionally, recombinant KRTAP5-11 serves as a tool in structural studies to elucidate interactions within the hair keratin network. Emerging applications include exploring its role in epithelial tissue repair and cosmetic science for developing hair-care products. Despite limited functional data compared to other KAPs, KRTAP5-11 remains a key target for understanding hair and skin pathophysiology, bridging gaps in genetic, molecular, and translational research.
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