| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRTAP3-2 |
| Uniprot No | Q9BYR7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-98aa |
| 活性数据 | MDCCASRSCSVPTGPATTICSSDKSCRCGVCLPSTCPHTVWLLEPICCDNCPPPCHIPQPCVPTCFLLNSCQPTPGLETLNLTTFTQPCCEPCLPSTI |
| 分子量 | 37.18 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KRTAP3-2蛋白的参考文献示例(注：内容为模拟生成，仅供参考)：
1. **文献名称**: "Expression and characterization of recombinant human KRTAP3-2 protein in E. coli"
**作者**: Liu, Y., et al.
**摘要**: 研究通过大肠杆菌系统表达了重组人KRTAP3-2蛋白，并优化了纯化条件，验证了其与角蛋白的相互作用，为其在毛发结构中的功能研究提供了基础。
2. **文献名称**: "KRTAP3-2 mutations alter hair keratin assembly in congenital hair shaft disorders"
**作者**: Smith, J.P., et al.
**摘要**: 发现KRTAP3-2基因突变通过破坏重组蛋白的二硫键形成，导致毛小皮结构异常，为遗传性毛发疾病提供了分子机制证据。
3. **文献名称**: "Structural analysis of human KRTAP3-2 and its role in modulating keratin network stability"
**作者**: Tanaka, R., et al.
**摘要**: 利用重组KRTAP3-2蛋白进行结构解析，揭示了其通过C末端结构域增强角蛋白纤维稳定性的机制，并提出了其在细胞机械强度中的作用。
4. **文献名称**: "Proteomic identification of KRTAP3-2 as a potential biomarker for alopecia areata"
**作者**: Wang, X., et al.
**摘要**: 通过蛋白质组学发现斑秃患者毛囊中KRTAP3-2表达显著下调，体外重组实验表明其缺失可能导致角蛋白网络脆性增加。
(提示：若需真实文献，建议通过PubMed或Google Scholar以关键词“KRTAP3-2 recombinant”检索。)
Recombinant human KRTAP3-2 protein is a engineered variant of the keratin-associated protein 3-2 (KRTAP3-2), a member of the keratin-associated protein (KAP) family. KAPs are critical components of hair and wool fibers, contributing to structural integrity, elasticity, and physicochemical properties by interacting with keratin intermediate filaments. KRTAP3-2. located within the human hair keratin-associated protein gene cluster on chromosome 17. is specifically expressed in the hair follicle cortex. Its cysteine-rich sequence enables disulfide bond formation, stabilizing the keratin matrix.
Recombinant KRTAP3-2 is typically produced via heterologous expression systems (e.g., E. coli or mammalian cells) for biochemical and functional studies. It lacks post-translational modifications unless expressed in eukaryotic systems. Research focuses on its role in hair shaft formation, genetic hair disorders (e.g., monilethrix or hypotrichosis), and interactions with keratins like K31 or K85. Its overexpression or mutations may disrupt hair fiber assembly, offering insights into pathogenic mechanisms. Additionally, recombinant KRTAP3-2 is utilized in material science for bioinspired polymers and in cosmetic research for hair-strengthening formulations. Current studies also explore its potential as a biomarker for hair-related diseases or nutritional deficiencies affecting keratinization.
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