| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRTAP4-4 |
| Uniprot No | Q9BYR3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-166aa |
| 活性数据 | MVNSCCGSVCSDQGCGLENCCRPSYCQTTCCRTTCCRPSCCVSSCCRPQCCQTTCCRTTCCHPSCCVSSCCRPQCCQSVCCQPTCCRPQCCQTTCCRTTCCRPSCCRPQCCQSVCCQPTCCCPSYCVSSCCRPQCCQTTCCRTTCCRPSCCVSRCYRPHCGQSLCC |
| 分子量 | 44.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KRTAP4-4蛋白的参考文献示例(注：以下内容为模拟生成，实际文献可能需要进一步检索验证)：
---
1. **"Expression and characterization of recombinant human KRTAP4-4 in Escherichia coli"**
*Authors: Smith JL, Wang Q, Liu H*
**摘要**：本研究报道了通过大肠杆菌系统高效表达重组人KRTAP4-4蛋白，优化了纯化条件并进行了SDS-PAGE及质谱鉴定，证实其正确折叠和稳定性，为后续功能研究奠定基础。
2. **"Functional analysis of KRTAP4-4 in hair follicle development using recombinant protein"**
*Authors: Zhang Y, Chen T, Li R*
**摘要**：利用重组KRTAP4-4蛋白进行体外毛囊细胞培养实验，发现其通过调节Wnt信号通路影响角质形成细胞分化，提示其在毛发结构形成中的关键作用。
3. **"Structural insights into KRTAP4-4 protein by cryo-EM and X-ray crystallography"**
*Authors: Gupta A, Kim S, Park J*
**摘要**：首次解析了重组KRTAP4-4蛋白的晶体结构和冷冻电镜三维模型，揭示了其与角蛋白结合的特定结构域，为理解其在皮肤屏障功能中的分子机制提供依据。
4. **"KRTAP4-4 mutations linked to hereditary skin disorders: Role of recombinant protein in pathogenicity screening"**
*Authors: Müller B, Schmidt D, Zhou X*
**摘要**：构建了携带致病性突变的重组KRTAP4-4蛋白，通过体外细胞模型证明突变导致蛋白聚集和细胞凋亡增加，为相关遗传性皮肤病的分子诊断提供新策略。
---
(注：以上文献信息为示例，实际引用请通过PubMed、Web of Science等平台查询最新研究。)
Recombinant human KRTAP4-4 protein is a engineered variant of the keratin-associated protein 4-4 (KRTAP4-4), a member of the keratin-associated protein (KAP) family. These proteins play critical roles in the structure and stability of hair and other keratinized tissues by interacting with keratin intermediate filaments. KRTAP4-4. encoded by the *KRTAP4-4* gene located on human chromosome 17q12. is specifically expressed in the hair follicle cortex and contributes to the formation of disulfide bond-rich matrix structures, which influence the mechanical properties of hair. Its cysteine-rich sequence facilitates cross-linking with keratins, enhancing tensile strength and elasticity.
The recombinant form is typically produced using bacterial or mammalian expression systems, enabling scalable purification for research and industrial applications. Studies focus on its potential in hair biology, dermatology, and tissue engineering, particularly in understanding hair disorders, designing biomaterials, or developing treatments for conditions like alopecia or brittle hair syndromes. However, functional mechanisms of KRTAP4-4 remain partially undefined, driving ongoing research to clarify its structural interactions and regulatory pathways. The recombinant protein’s availability supports functional assays, antibody development, and high-throughput screening for therapeutic innovations. Challenges include maintaining proper disulfide bonding during in vitro production to ensure native-like activity.
×
