| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRTAP19-4 |
| Uniprot No | Q3LI73 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-84aa |
| 活性数据 | MSYYGSYYRGLGYGCGGFGGLGYGYGCGCGSFRRLGYGCGFGGNGYGYCRPSCYGGYGFSILLKSYPEDTISEVIRRSFNLTKY |
| 分子量 | 9.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KRTAP19-4蛋白的参考文献示例(注：由于该蛋白研究较为专业且文献有限，以下内容为结合相关领域研究的模拟示例，实际文献需通过学术数据库进一步验证)：
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1. **文献名称**: *"Characterization of the human keratin-associated protein 19-4 gene and its recombinant expression in vitro"*
**作者**: Smith J, et al.
**摘要**: 研究首次报道了KRTAP19-4基因的克隆及在原核系统(如大肠杆菌)中的重组表达，验证了该蛋白的分子量(约10 kDa)及富含半胱氨酸的结构特征，表明其在毛发角蛋白交联中的潜在作用。
2. **文献名称**: *"Functional analysis of recombinant human KRTAP19-4 in hair shaft integrity using a transgenic mouse model"*
**作者**: Li X, et al.
**摘要**: 通过重组KRTAP19-4蛋白体外结合实验及转基因小鼠模型，发现该蛋白与毛发角蛋白(如KRT85)存在相互作用，缺失或突变会导致毛发机械强度下降，提示其对毛发结构的重要性。
3. **文献名称**: *"Proteomic identification of KRTAP19-4 as a novel biomarker in human scalp disorders"*
**作者**: Tanaka M, et al.
**摘要**: 研究通过重组KRTAP19-4蛋白制备抗体，结合质谱分析发现该蛋白在雄激素性脱发患者毛囊中表达显著下调，表明其可能作为脱发相关疾病的潜在生物标志物。
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**建议**：若需真实文献，请通过 **PubMed**、**Google Scholar** 或 **Web of Science** 检索关键词：
`recombinant KRTAP19-4`、`KRTAP19-4 expression`、`keratin-associated protein 19-4 function`。
Keratins and keratin-associated proteins (KAPs) are critical structural components of hair and wool fibers, contributing to their mechanical strength and elasticity. The human keratin-associated protein 19-4 (KRTAP19-4) belongs to the high sulfur KAP family, encoded by genes clustered in the human chromosome 21q22.1 region. This protein is specifically expressed in the hair follicle cortex, where it interacts with keratin intermediate filaments through disulfide bonds to stabilize the hair shaft structure. Variations in KRTAP genes, including KRTAP19-4. are linked to phenotypic diversity in hair texture, strength, and susceptibility to hair disorders.
Recombinant human KRTAP19-4 is synthesized via heterologous expression systems (e.g., E. coli or mammalian cells) for functional studies. Its production enables research into molecular interactions within keratin networks, sulfur-rich domain functionalities, and mechanisms influencing hair fiber properties. Studies on recombinant KRTAP19-4 also explore its role in hair disorders like monilethrix or hypotrichosis, aiming to develop therapeutic or cosmetic interventions.
Despite its niche focus, KRTAP19-4 exemplifies how keratin-associated proteins bridge genetic diversity and biomechanical traits, offering insights into evolutionary biology and biomaterial engineering. Current research emphasizes structure-function relationships and potential applications in bio-inspired materials or hair care innovations.
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