| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRTAP19-1 |
| Uniprot No | Q8IUB9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-90aa |
| 活性数据 | MSHYGSYYGGLGYSCGGFGGLGYGYGCGCGSFCRRGSGCGYGGYGYGSGFGSYGYGSGFGGYGYGSGFGGYGYGCCRPSYNGGYGFSGFY |
| 分子量 | 9.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是几篇关于重组人KRTAP19-1蛋白的模拟参考文献示例(内容为虚构，供参考)：
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1. **文献名称**：《重组人KRTAP19-1蛋白的克隆表达及结构特征分析》
**作者**：张磊, 王明, 李娟
**摘要**：本研究通过基因克隆技术将人源KRTAP19-1基因转入大肠杆菌表达系统，成功实现重组蛋白的高效表达与纯化。利用圆二色谱和质谱分析表明，重组蛋白具有典型的角蛋白相关蛋白α螺旋结构，可能参与毛发角化过程的功能调控。
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2. **文献名称**：《KRTAP19-1在人类毛囊发育中的表达及其突变关联性研究》
**作者**：Li, X., Chen, Y., Wang, H.
**摘要**：通过免疫组化和Western blot检测KRTAP19-1在毛囊中的特异性表达，结合基因测序发现其突变与遗传性毛发稀疏症显著相关。重组蛋白的功能实验揭示其通过调控角蛋白交联影响毛干强度。
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3. **文献名称**：《Comparative genomics and evolutionary analysis of the KRTAP19-1 gene family in mammals》
**作者**：Smith, J., Brown, K., García, R.
**摘要**：通过比较基因组学分析多个哺乳动物物种的KRTAP19-1同源基因，发现其高度保守的启动子区域可能调控毛发特异性表达。重组蛋白的功能进化研究表明，该蛋白在物种间适应不同环境压力中发挥关键作用。
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4. **文献名称**：《重组KRTAP19-1蛋白与角蛋白相互作用的功能研究》
**作者**：王芳, 刘伟, 陈涛
**摘要**：利用Pull-down实验和表面等离子体共振技术(SPR)证明KRTAP19-1与角蛋白K85在体外直接结合。重组蛋白的过表达可增强上皮细胞机械强度，提示其潜在应用于毛发再生治疗。
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**注意**：以上文献信息均为模拟创作，实际研究需通过学术数据库(如PubMed、Web of Science)检索真实文献。
Keratin-associated protein 19-1 (KRTAP19-1) is a member of the keratin-associated protein (KAP) family, which plays a critical role in the structural integrity and mechanical properties of hair and skin. KAPs interact with keratin intermediate filaments to form a resilient matrix, contributing to the strength, elasticity, and stability of epithelial tissues. Human KRTAP19-1 is encoded by the *KRTAP19-1* gene, located within a cluster of KAP genes on chromosome 21q22.1. This gene family exhibits high polymorphism and genetic variability, potentially influencing phenotypic diversity in hair texture and disease susceptibility.
Recombinant human KRTAP19-1 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional studies. Its production typically involves cloning the coding sequence into expression vectors, followed by purification via affinity chromatography. Recombinant KRTAP19-1 serves as a tool to investigate keratin network assembly, cellular stress responses, and interactions with other structural proteins. It also aids in exploring pathogenic mechanisms in keratin-related disorders, such as monilethrix or pachyonychia congenita, and may support drug screening for therapeutic interventions. Despite limited direct characterization of KRTAP19-1. its homology to well-studied KAPs suggests roles in redox regulation, apoptosis modulation, and barrier function, highlighting its broader relevance in dermatology and epithelial biology research.
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