| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRTAP1-5 |
| Uniprot No | Q9BYS1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-174aa |
| 活性数据 | MTCCQTSFCGYPSFSISGTCGSSCCQPSCCETSCCQPRSCQTSFCGFPSFSTSGTCSSSCCQPSCCETSCCQPSCCETSCCQPSCCQISSCGTGCGIGGGISYGQEGSSGAVSTRIRWCRPDSRVEGTYLPPCCVVSCTPPSCCQLHHAQASCCRPSYCGQSCCRPVCCCEPTC |
| 分子量 | 44.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KRTAP1-5蛋白的模拟参考文献(基于合理推测)：
1. **《Expression and Characterization of Recombinant Human KRTAP1-5 in Escherichia coli》**
- **作者**: Zhang, L. et al.
- **摘要**: 本研究成功克隆人源KRTAP1-5基因，并利用大肠杆菌表达系统实现可溶性重组蛋白的高效表达，通过亲和层析纯化后证实其与天然蛋白相似的二级结构及生物学活性。
2. **《Role of KRTAP1-5 in Hair Shaft Strength: Insights from Recombinant Protein Analysis》**
- **作者**: Smith, J.R. & Wang, Y.
- **摘要**: 通过重组KRTAP1-5蛋白的功能实验，发现其通过调控角蛋白交联影响毛发纤维的机械强度，为毛发脆弱性疾病的分子机制提供新依据。
3. **《Structural Insights into KRTAP1-5 Interactions via X-ray Crystallography》**
- **作者**: Chen, H. et al.
- **摘要**: 首次解析重组KRTAP1-5蛋白的晶体结构，揭示其N端结构域与角蛋白K38的特异性结合位点，为理解皮肤屏障功能的分子基础提供结构数据。
4. **《KRTAP1-5 Genetic Variants and Their Recombinant Protein Implications in Ectodermal Dysplasia》**
- **作者**: Gupta, S. et al.
- **摘要**: 分析KRTAP1-5基因突变与外胚层发育不良的关联，体外实验表明突变型重组蛋白破坏角蛋白网络组装，提示其病理作用机制。
(注：以上文献为模拟示例，实际研究需查阅具体数据库如PubMed。)
KRTAP1-5 (Keratin-Associated Protein 1-5) is a member of the keratin-associated protein (KAP) family, which plays critical roles in hair and nail formation by interacting with keratin filaments to form stable cytoskeletal networks. These proteins are enriched in cysteine residues, enabling disulfide bond formation that strengthens hair shaft integrity and elasticity. As part of the KRTAP1 cluster on human chromosome 17. KRTAP1-5 is specifically expressed in the hair follicle cortex, contributing to the matrix structure of keratinocytes during differentiation.
Recombinant human KRTAP1-5 is produced via genetic engineering, often using bacterial or mammalian expression systems, to study its biochemical properties and interactions. Its recombinant form allows researchers to explore structural-functional relationships, such as how cysteine patterning influences cross-linking efficiency and mechanical resilience in hair. Dysregulation or mutations in KRTAP genes are linked to hereditary hair disorders like monilethrix and hypotrichosis, making this protein a focus for understanding disease mechanisms. Additionally, recombinant KRTAP1-5 serves as a tool for developing biomimetic materials or therapies targeting hair-related pathologies. Ongoing research aims to elucidate its precise role in keratin organization and potential applications in regenerative medicine or cosmetic biotechnology.
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