| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIAA0971 |
| Uniprot No | Q9NYY8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-710aa |
| 活性数据 | MLTTLKPFGS VSVESKMNNK AGSFFWNLRQ FSTLVSTSRT MRLCCLGLCK PKIVHSNWNI LNNFHNRMQS TDIIRYLFQD AFIFKSDVGF QTKGISTLTA LRIERLLYAK RLFFDSKQSL VPVDKSDDEL KKVNLNHEVS NEDVLTKETK PNRISSRKLS EECNSLSDVL DAFSKAPTFP SSNYFTAMWT IAKRLSDDQK RFEKRLMFSH PAFNQLCEHM MREAKIMQYK YLLFSLHAIV KLGIPQNTIL VQTLLRVTQE RINECDEICL SVLSTVLEAM EPCKNVHVLR TGFRILVDQQ VWKIEDVFTL QVVMKCIGKD APIALKRKLE MKALRELDRF SVLNSQHMFE VLAAMNHRSL ILLDECSKVV LDNIHGCPLR IMINILQSCK DLQYHNLDLF KGLADYVAAT FDIWKFRKVL FILILFENLG FRPVGLMDLF MKRIVEDPES LNMKNILSIL HTYSSLNHVY KCQNKEQFVE VMASALTGYL HTISSENLLD AVYSFCLMNY FPLAPFNQLL QKDIISELLT SDDMKNAYKL HTLDTCLKLD DTVYLRDIAL SLPQLPRELP SSHTNAKVAE VLSSLLGGEG HFSKDVHLPH NYHIDFEIRM DTNRNQVLPL SDVDTTSATD IQRVAVLCVS RSAYCLGSSH PRGFLAMKMR HLNAMGFHVI LVNNWEMDKL EMEDAVTFLK TKIYSVEALP VAAVNVQSTQ |
| 分子量 | 81.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KIAA0971蛋白的模拟参考文献示例，包含文献名称、作者及摘要概括：
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1. **文献名称**：*Molecular Cloning and Recombinant Expression of Human KIAA0971 Protein in HEK293 Cells*
**作者**：Tanaka Y, et al.
**摘要**：该研究报道了通过PCR克隆人KIAA0971基因，并在HEK293细胞中进行重组表达。作者纯化了重组蛋白并验证其跨膜结构域，发现其可能参与细胞内囊泡运输过程。
2. **文献名称**：*KIAA0971 Regulates Autophagy via Interaction with ATG16L1 in Human Colorectal Cancer Cells*
**作者**：Suzuki M, et al.
**摘要**：本研究利用重组表达的KIAA0971蛋白，通过免疫共沉淀实验发现其与自噬关键蛋白ATG16L1相互作用，抑制KIAA0971表达可显著降低癌细胞自噬活性。
3. **文献名称**：*Structural and Functional Analysis of Recombinant KIAA0971 Using Cryo-EM*
**作者**：Wang X, et al.
**摘要**：作者在大肠杆菌中高效表达并纯化重组人KIAA0971蛋白，结合冷冻电镜技术解析其三维结构，揭示其N端结构域可能介导蛋白复合物形成。
4. **文献名称**：*KIAA0971 Promotes Tumor Angiogenesis through VEGF Signaling Pathway*
**作者**：Zhang L, et al.
**摘要**：通过重组KIAA0971蛋白的体外实验，研究发现其能增强内皮细胞迁移和血管生成素(VEGF)分泌，提示其在肿瘤血管生成中的潜在作用。
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**注**：以上文献为示例性质，实际研究中可能需要根据具体数据库检索调整关键词或核实最新进展。
**Background of Recombinant Human KIAA0971 Protein**
The human KIAA0971 gene, also known as FAM71E1. encodes a protein belonging to the FAM71 family, which comprises poorly characterized proteins with potential roles in cellular processes. KIAA0971 is predominantly expressed in the testis, suggesting involvement in reproductive biology, particularly spermatogenesis or sperm function. Structurally, it contains predicted coiled-coil domains, indicative of protein-protein interaction capabilities, though its precise molecular mechanisms remain unclear.
Recombinant KIAA0971 protein is engineered for in vitro studies to elucidate its function. Produced using expression systems like *E. coli* or mammalian cells (e.g., HEK293), the recombinant form enables investigations into its biochemical properties, interactome, and post-translational modifications. Recent studies propose its association with sperm motility and acrosome reaction, potentially linking it to male infertility. However, its broader physiological or pathological relevance in non-reproductive tissues is yet unexplored.
Research on recombinant KIAA0971 also supports antibody development for diagnostic applications or cellular localization studies. Despite its potential biomedical significance, direct disease associations remain unestablished, highlighting the need for further functional and clinical research. Current efforts focus on deciphering its role in signaling pathways or structural complexes, leveraging recombinant protein tools to bridge knowledge gaps in its contribution to cellular homeostasis or reproductive health.
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