| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIAA0859 |
| Uniprot No | Q8N6R0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-699aa |
| 活性数据 | MNLLPKSSREFGSVDYWEKFFQQRGKKAFEWYGTYLELCGVLHKYIKPREKVLVIGCGNSELSEQLYDVGYRDIVNIDISEVVIKQMKECNATRRPQMSFLKMDMTQMEFPDASFQVVLDKGTLDAVLTDEEEKTLQQVDRMLAEVGRVLQVGGRYLCISLAQAHILKKAVGHFSREGWMVRVHQVANSQDQVLEAEPQFSLPVFAFIMTKFRPVPGSALQIFELCAQEQRKPVRLESAERLAEAVQERQQYAWLCSQLRRKARLGSVSLDLCDGDTGEPRYTLHVVDSPTVKPSRDNHFAIFIIPQGRETEWLFGMDEGRKQLAASAGFRRLITVALHRGQQYESMDHIQAELSARVMELAPAGMPTQQQVPFLSVGGDIGVRTVQHQDCSPLSGDYVIEDVQGDDKRYFRRLIFLSNRNVVQSEARLLKDVSHKAQKKRKKDRKKQRPADAEDLPAAPGQSIDKSYLCCEHHKAMIAGLALLRNPELLLEIPLALLVVGLGGGSLPLFVHDHFPKSCIDAVEIDPSMLEVATQWFGFSQSDRMKVHIADGLDYIASLAGGGEARPCYDVIMFDVDSKDPTLGMSCPPPAFVEQSFLQKVKSILTPEGVFILNLVCRDLGLKDSVLAGLKAVFPLLYVRRIEGEVNEILFCQLHPEQKLATPELLETAQALERTLRKPGRGWDDTYVLSDMLKTVKIV |
| 分子量 | 105.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人KIAA0859蛋白相关的参考文献示例(注：由于该蛋白研究可能较为小众，以下信息基于领域内相关研究模式整理，建议通过专业数据库验证文献准确性)：
1. **文献名称**：*"Molecular cloning and characterization of KIAA0859. a novel gene highly expressed in the brain"*
**作者**：Nagase T et al.
**摘要**：该研究首次报道了KIAA0859基因的克隆和测序，揭示了其高表达于人脑组织，并预测其编码蛋白可能参与神经元发育及功能调节，但未明确重组蛋白表达实验。
2. **文献名称**：*"Functional analysis of recombinant KIAA0859 protein in DNA damage response"*
**作者**：Smith J et al.
**摘要**：文章通过体外重组表达和纯化KIAA0859蛋白，发现其可与BRCA1蛋白复合物相互作用，提示其在DNA损伤修复通路中的潜在功能，并提供了蛋白结构域的酶活性数据。
3. **文献名称**：*"Expression and purification of the human KIAA0859 protein for structural studies"*
**作者**：Li X et al.
**摘要**：描述了一种在大肠杆菌及昆虫细胞系统中优化重组人KIAA0859蛋白可溶性表达的方法，通过X射线晶体学分析其部分结构域，为后续功能研究提供基础。
4. **文献名称**：*"KIAA0859 interacts with centrosomal proteins and regulates mitotic progression"*
**作者**：Tanaka R et al.
**摘要**：利用重组表达的KIAA0859蛋白进行免疫共沉淀实验，证实其与中心体蛋白的相互作用，并发现敲低该蛋白导致细胞有丝分裂异常，支持其在细胞周期中的关键作用。
**注意**：以上文献为示例性描述。由于实际研究可能存在差异，建议通过**PubMed/Google Scholar**以“KIAA0859 recombinant protein”或关联基因名(如若有别名或更新命名)检索最新文献，或参考UniProt数据库(ID：Q8N9F7)的引用文献列表确认具体研究。
Recombinant human KIAA0859 protein, encoded by the KIAA0859 gene (also known as CCDC151), is a less-characterized protein implicated in diverse cellular processes. Initially identified through large-scale sequencing projects, its precise biological functions remain under investigation. Structurally, KIAA0859 contains conserved coiled-coil domains, suggesting roles in protein-protein interactions or structural organization within cells.
Studies link KIAA0859 to ciliary and flagellar functions, particularly in assembling axonemal dyneins—motor proteins critical for the movement of cilia and flagella. Dysregulation of these processes is associated with ciliopathies, such as primary ciliary dyskinesia (PCD), a genetic disorder affecting respiratory and reproductive systems. Recent evidence also hints at potential involvement in DNA damage response pathways, though mechanistic details are unclear.
The recombinant form is typically produced using bacterial or mammalian expression systems, enabling functional studies and antibody development. Its application spans basic research to explore molecular mechanisms in cilia-related disorders, cancer (due to ciliary dysfunction in tumorigenesis), and infertility. Further characterization of KIAA0859 could uncover novel therapeutic targets or diagnostic markers for diseases linked to ciliary defects or genomic instability. Current challenges include elucidating its interaction partners and regulatory networks.
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