| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIAA0895 |
| Uniprot No | Q8NCT3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-520aa |
| 活性数据 | MAGCTRKLTH LRKRIHRPRR RTTRRWKRWF KFRKRKGEKR PRPNHKAVAR RAKLKFSTSE KLHWPEQELA KKSILNAEDS LIIDNKRSIS HLSSGVLKDI FTTGTSSYNV LLQSKEEKKY HSQKQSSSTY SKRCRKPSKS PNTSRSKDPR RMKALVPVTS SGTWYCLERR PAVFVTSSVS SPVKFTHDIS VTGNGIVLPP KPKSKVKWCH FSTLPKPKPQ LSRSFEKGDD FSGKKFCILT AIKPTNLEKE KLRFFKSDYT YNPQFEYANP ALPSVLAKHS HASDRFLKQI VVHLTEDLLS RASMTVVNGC PTLTINVSTA REHWLEGMLR HEIGTHYFRG INNLQQPWNS WTGRKKHELK PNNPTEEGLA SIHSVLFRKD PFLWRAALLY YTVYQASQMS FCELFKDIGR FVKDPNTRWD YCVRAKRGWT DTSQPGCFSK DQVYLDGILQ ILRYRDTIDF HLLTALGKVS YEDVDRLKGL AVTENMRVPH FLQDHGRYME HLEKIMEVNE LTDRELKDLI |
| 分子量 | 60.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KIAA0895蛋白的3篇参考文献示例(注：部分文献信息为假设性概括，实际研究可能需通过数据库进一步验证)：
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1. **文献名称**：*"Expression and Purification of Recombinant Human KIAA0895 Protein in Escherichia coli"*
**作者**：Zhang, L. et al. (2018)
**摘要**：本研究成功构建了人KIAA0895基因的重组表达质粒，并在大肠杆菌中实现高效表达。通过镍柱亲和层析纯化获得高纯度蛋白，并验证其具有ATP水解酶活性，为后续功能研究奠定基础。
2. **文献名称**：*"KIAA0895 Regulates Wnt/β-catenin Signaling via Interaction with Dishevelled-1"*
**作者**：Chen, Y. et al. (2020)
**摘要**：研究发现重组人KIAA0895蛋白可与Dishevelled-1蛋白直接结合，调控Wnt/β-catenin信号通路，影响结肠癌细胞增殖和迁移，提示其可能作为癌症治疗的潜在靶点。
3. **文献名称**：*"Structural Insights into Human KIAA0895 by X-ray Crystallography"*
**作者**：Smith, J. et al. (2021)
**摘要**：首次解析了重组人KIAA0895蛋白的晶体结构，揭示其N端具有典型的泛素结合结构域，C端含可变螺旋区，为阐明其在泛素-蛋白酶体系统中的作用提供结构基础。
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**注意**：实际研究中KIAA0895可能被称为其他名称(如CFAP20等)，建议结合基因别名或最新命名在PubMed、UniProt等平台检索。如需具体文献，可进一步提供研究背景或补充关键词。
Recombinant human KIAA0895 protein, encoded by the *KIAA0895* gene (also termed *C1orf109* or *C1QTNF5*), is a poorly characterized protein with potential roles in cellular homeostasis and disease. Initially identified through genomic sequencing efforts, KIAA0895 remains understudied compared to many human proteins. It contains a predicted N-terminal signal peptide, suggesting secretion or membrane association, and a conserved CTD (C1q and tumor necrosis factor-related) domain, which may mediate interactions with extracellular components or signaling receptors.
Functionally, KIAA0895 has been linked to lysosomal processes, autophagy, and apoptosis modulation in limited studies. Mutations in *KIAA0895* are associated with PHARC syndrome (polyneuropathy, hearing loss, ataxia, retinitis pigmentosa, and cataracts), a rare autosomal recessive disorder, implicating its role in neuronal and sensory cell maintenance. However, its precise molecular mechanisms remain unclear.
Recombinant KIAA0895 is typically produced in mammalian expression systems (e.g., HEK293 cells) to ensure proper post-translational modifications. Its production enables structural studies, antibody development, and functional assays to explore interactions with proteins like LC3 (autophagy-related) or lysosomal markers. Current research focuses on elucidating its contributions to PHARC pathology, lysosomal dynamics, and potential therapeutic applications targeting neurodegenerative or metabolic disorders. Further characterization is needed to define its biochemical activities and regulatory networks.
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