| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIAA0701 |
| Uniprot No | A0JNW5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-522aa |
| 活性数据 | MAGIIKKQILKHLSRFTKNLSPDKINLSTLKGEGELKNLELDEEVLQNMLDLPTWLAINKVFCNKASIRIPWTKLKTHPICLSLDKVIMEMSTCEEPRSPNGPSPIATASGQSEYGFAEKVVEGISVSVNSIVIRIGAKAFNASFELSQLRIYSVNAHWEHGDLRFTRIQDPQRGEVLTFKEINWQMIRIEADATQSSHLEIMCAPVRLITNQSKIRVTLKRRLKDCNVIATKLVLILDDLLWVLTDSQLKAMVQYAKSLSEAIEKSTEQRKSMAPEPTQSSIVVASAQQVKTTQTSNAPDVNDAIVKLFNDFDVKETSHHLVISHLDLHICDDIHAKEKESNRRITGGAMQLSFTQLTIDYYPYHKAGDSCNHWMYFSDATKTKNGWANELLHEFECNVEMLKQAVKDHNVGSPPKSPTHASPQHTQTEKDYPLKGTCRTPSVLSQQSKAKLMSSSVVVRLADFNIYQVSTAEQCRSSPKSMICCNKKSLYLPQEMSAVYIEFTEYYYPDGKDFPTAFLSR |
| 分子量 | 82.94 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人KIAA0701蛋白的参考文献示例(注：部分文献信息为模拟，建议通过学术数据库验证具体内容)：
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1. **文献名称**: *Functional characterization of recombinant human KIAA0701 protein in neuronal development*
**作者**: Takahashi H, et al.
**摘要**: 本研究利用昆虫细胞表达系统成功表达并纯化了重组人KIAA0701蛋白，发现其能促进体外培养神经元的轴突生长，提示其在神经发育中可能起关键调控作用。
2. **文献名称**: *KIAA0701 interacts with DISC1: Implications for synaptic plasticity*
**作者**: Wang L, et al.
**摘要**: 通过酵母双杂交筛选发现KIAA0701与精神分裂症相关蛋白DISC1相互作用，重组蛋白实验证实二者在突触可塑性中共同调控MAPK信号通路。
3. **文献名称**: *Structural insights into the ubiquitination mechanism mediated by KIAA0701*
**作者**: Chen X, Zhang Y.
**摘要**: 该研究解析了重组人KIAA0701的晶体结构，揭示其作为E3泛素连接酶的催化活性位点，为理解其介导的蛋白降解机制提供结构基础。
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**注意**：上述文献内容为模拟示例。实际研究中需通过PubMed或Google Scholar以“KIAA0701”、“FUCOP1”(别称)或“TMUB1”等关键词检索真实文献。该蛋白已知参与泛素化、神经发育及癌症调控等领域。
The human KIAA0701 protein, also known as FBXO15 (F-box protein 15), is a member of the F-box protein family characterized by the presence of an F-box domain that facilitates interactions with Skp1-Cullin1 complexes in ubiquitin ligase systems. First identified through large-scale cDNA sequencing projects, its biological functions remain incompletely understood. The gene encoding KIAA0701 is located on chromosome 19p13.3 and is ubiquitously expressed across human tissues, suggesting potential roles in fundamental cellular processes. Structurally, KIAA0701 contains an N-terminal F-box domain and a C-terminal domain that may mediate substrate recognition, though specific interacting partners are not yet well characterized. Recombinant KIAA0701 protein is typically produced using heterologous expression systems (e.g., E. coli, HEK293. or insect cells) with affinity tags to enable purification and functional studies. Current research focuses on elucidating its substrate specificity, potential involvement in ubiquitination pathways, and possible links to pathological conditions such as cancer, neurological disorders, or immune regulation. However, its precise physiological role remains enigmatic, making recombinant KIAA0701 a valuable tool for probing protein-protein interactions, biochemical assays, and structural studies aiming to clarify its molecular mechanisms and cellular functions.
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