| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIAA0495 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-201aa |
| 活性数据 | MCLLSSSAASDLAATSLTAHPCHPPLPRQPQPQLILSGSPSIGTVPPPAVGHTSLLPVNPPAISTTTSDLSAREDATPSASTGHLSVFPAFQVKIPAVPSEQTSQSFSEASHRVLPGGGPRCSRDFGAGVAGHLGLGIFGVGFGSPALLQSVVDENSCCLLYVVEDQLCDVEQAFRAEHLGQHQSGSGTGCRHCPQRPAAL |
| 分子量 | 47 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Characterization of Recombinant KIAA0495 Protein in Age-Related Macular Degeneration"**
- Authors: Smith J, et al.
- 摘要：研究利用重组人KIAA0495蛋白，通过体外功能实验验证其与HTRA1的相互作用，发现其在AMD病理中可能调控细胞外基质降解，为疾病机制提供新见解。
2. **"Expression and Structural Analysis of Recombinant KIAA0495/ARMS2 in Eukaryotic Systems"**
- Authors: Chen L, et al.
- 摘要：通过哺乳动物表达系统成功表达重组KIAA0495蛋白，分析其结构特征并揭示其热休克蛋白结构域的功能，提示其在氧化应激反应中的潜在作用。
3. **"Functional Role of Recombinant KIAA0495 in Neuronal Development"**
- Authors: Müller R, et al.
- 摘要：利用重组蛋白探究KIAA0495对神经元突触形成的调控作用，发现其通过调节Wnt信号通路促进轴突生长，为神经发育研究提供实验基础。
4. **"Proteomic Profiling of KIAA0495 Interactions Using Recombinant Protein Pull-Down Assays"**
- Authors: Kim T, et al.
- 摘要：构建重组KIAA0495蛋白进行Pull-Down联合质谱分析，鉴定出多个新的结合蛋白，包括线粒体代谢相关因子，提示其参与能量代谢调控。
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注：以上文献为示例性质，实际研究中建议通过PubMed或Web of Science以“KIAA0495”“ARMS2”“recombinant protein”为关键词检索最新论文。部分研究可能以疾病相关名称(如AMD)为主要标题。
Recombinant human KIAA0495 protein is derived from the KIAA0495 gene, initially identified through large-scale sequencing projects focused on mapping uncharted regions of the human genome. The gene, also termed EML1 (Echinoderm Microtubule-associated protein-like 1), encodes a microtubule-associated protein predominantly expressed in the brain, particularly during early neuronal development. KIAA0495/EML1 belongs to the EMAP family, characterized by conserved tandem WD40-repeat domains that facilitate protein-protein interactions and microtubule stabilization. Dysregulation of this protein has been linked to neurodevelopmental disorders, including cortical malformations, heterotopia, and epilepsy, highlighting its role in neuronal migration and cortical layering.
Recombinant KIAA0495 is typically produced in vitro using expression systems like E. coli or mammalian cells, enabling functional studies to decipher its molecular mechanisms. Its recombinant form allows researchers to explore its interaction with cytoskeletal components, cell cycle regulators, and signaling pathways. Studies suggest it may influence mitotic spindle orientation, axon guidance, and synaptic plasticity. Additionally, recombinant KIAA0495 serves as a tool for antibody development, drug screening, and modeling pathological mutations associated with brain disorders. Despite its established role in neurodevelopment, many aspects of its structure-function relationships and broader physiological impacts remain under investigation, underscoring its potential as a therapeutic target or biomarker.
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