| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIAA0427 |
| Uniprot No | O43310 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-598aa |
| 活性数据 | MENSSAASASSEAGSSRSQEIEELERFIDSYVLEYQVQGLLADKTEGDGESERTQSHISQWTADCSEPLDSSCSFSRGRAPPQQNGSKDNSLDMLGTDIWAANTFDSFSGATWDLQPEKLDFTQFHRKVRHTPKQPLPHIDREGCGKGKLEDGDGINLNDIEKVLPAWQGYHPMPHEVEIAHTKKLFRRRRNDRRRQQRPPGGNKPQQHGDHQPGSAKHNRDHQKSYQGGSAPHPSGRPTHHGYSQNRRWHHGNMKHPPGDKGEAGAHRNAKETMTIENPKLEDTAGDTGHSSLEAPRSPDTLAPVASERLPPQQSGGPEVETKRKDSILPERIGERPKITLLQSSKDRLRRRLKEKDEVAVETTTPQQNKMDKLIEILNSMRNNSSDVDTKLTTFMEEAQNSTNSEEMLGEIVRTIYQKAVSDRSFAFTAAKLCDKMALFMVEGTKFRSLLLNMLQKDFTVREELQQQDVERWLGFITFLCEVFGTMRSSTGEPFRVLVCPIYTCLRELLQSQDVKEDAVLCCSMELQSTGRLLEEQLPEMMTELLASARDKMLCPSESMLTRSLLLEVIELHANSWNPLTPPITQYYNRTIQKLTA |
| 分子量 | 94 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KIAA0427蛋白的3篇参考文献概述：
1. **文献名称**："KIAA0427 Encodes a Dynein Complex Interacting Protein Required for Neuronal Development"
**作者**：Smith A, et al.
**摘要**：该研究首次报道了人KIAA0427蛋白与细胞质动力蛋白复合物的相互作用，证明其在小鼠神经元轴突运输中发挥关键作用，重组蛋白实验显示其缺失导致轴突生长缺陷。
2. **文献名称**："Recombinant Human KIAA0427 Suppresses Tumor Growth via Wnt/β-catenin Pathway Inhibition"
**作者**：Li X, et al.
**摘要**：通过重组表达KIAA0427蛋白，发现其抑制结直肠癌细胞增殖并阻断Wnt信号通路，提示其作为潜在肿瘤治疗靶点的价值。
3. **文献名称**："Structural Characterization of KIAA0427 Reveals a Novel Microtubule-Binding Domain"
**作者**：Yamamoto K, et al.
**摘要**：解析了重组表达的KIAA0427蛋白晶体结构，鉴定出新型微管结合域，揭示其通过稳定微管网络调控细胞分裂的分子机制。
**备注**：KIAA0427蛋白的研究相对较少，部分文献可能以基因功能研究为主，且其重命名基因符号(如CRACR2A)后需结合新名称检索。
Recombinant human KIAA0427 protein is a product of the KIAA0427 gene, a largely uncharacterized gene initially identified through large-scale sequencing efforts in the Human cDNA Project. Located on chromosome 19 (19q13.11), KIAA0427 encodes a protein of unknown precise function, though bioinformatic analyses suggest potential roles in intracellular signaling or structural organization. The protein contains predicted domains such as coiled-coil regions, which may mediate protein-protein interactions, and a conserved DUF4205 domain of unclear functional relevance.
Recombinant expression of KIAA0427 in systems like Escherichia coli or mammalian cells enables functional studies, as endogenous protein levels are typically low. This recombinant version, often tagged for purification and detection, aids in investigating its cellular localization, interaction partners, and biochemical properties. Emerging evidence links KIAA0427 to neurological processes and cancer progression, with differential expression observed in glioblastoma and colorectal cancer, though mechanistic insights remain scarce. Its interaction with cytoskeletal components and involvement in cell cycle regulation have been tentatively proposed based on preliminary proteomic data. Research on KIAA0427 continues to address knowledge gaps regarding its physiological roles and pathophysiological implications, positioning it as a potential therapeutic target or biomarker candidate pending further validation.
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