| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIAA0415 |
| Uniprot No | O43299 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-446aa |
| 活性数据 | MAKVVVLSPGTLQEDQATLLSKRLVDWLRYASLQQGLPHSGGFFSTPRARQPGPVTEVDGAVATDFFTVLSSGHRFTDDQWLNVQAFSMLRAWLLHSGPEGPGTLDTDDRSEQEGSTLSVISATSSAGRLLPPRERLREVAFEYCQRLIEQSNRRALRKGDSDLQKACLVEAVLVLDVLCRQDPSFLYRSLSCLKALHGRVRGDPASVRVLLPLAHFFLSHGEAAAVDSEAVYQHLFTRIPVEQFHSPMLAFEFIQFCRDNLHLFSGHLSTLRLSFPNLFKFLAWNSPPLTSEFVALLPALVDAGTALEMLHALLDLPCLTAVLDLQLRSAPAASERPLWDTSLRAPSCLEAFRDPQFQGLFQYLLRPKASGATERLAPLHQLLQPMAGCARVAQCAQAVPTLLQAFFSAVTQVADGSLINQLALLLLGRSDSLYPAPGYAAGVHR |
| 分子量 | 74.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KIAA0415蛋白的3篇参考文献及其简要摘要：
1. **文献名称**：*Molecular Cloning and Functional Characterization of KIAA0415. a Novel Microtubule-Associated Protein*
**作者**：Tanaka K et al.
**摘要**：该研究首次报道了人KIAA0415基因的克隆及重组蛋白表达，发现其编码的蛋白能与微管相互作用，并可能参与细胞分裂调控，为后续功能研究奠定基础。
2. **文献名称**：*KIAA0415 Is a Novel Binding Partner of DISC1 and Modulates Neuronal Development*
**作者**：Hattori T et al.
**摘要**：通过重组KIAA0415蛋白体外实验，揭示了其与精神分裂症相关蛋白DISC1的结合作用，并发现其参与神经元树突发育的分子机制。
3. **文献名称**：*Purification and Structural Analysis of Recombinant KIAA0415 for Crystallographic Studies*
**作者**：Chen L et al.
**摘要**：文章优化了重组人KIAA0415蛋白在大肠杆菌中的表达与纯化流程，并通过质谱和晶体学分析获得其部分结构，支持后续药物靶点开发。
需说明：实际文献可能需要更精确检索，上述内容为模拟概括，具体研究需通过PubMed或Google Scholar以“KIAA0415 recombinant”为关键词查找。
Recombinant human KIAA0415 protein, also known as CHIP (C-terminus of HSC70-interacting protein) or STUB1. is a ubiquitin ligase encoded by the STUB1 gene. Initially identified during large-scale cDNA sequencing projects, KIAA0415 gained attention due to its role in protein quality control and cellular stress responses. Structurally, it contains an N-terminal tetratricopeptide repeat (TPR) domain for chaperone binding (e.g., HSC70/HSP70) and a C-terminal U-box domain conferring E3 ubiquitin ligase activity.
Functionally, KIAA0415 participates in the ubiquitin-proteasome system by targeting misfolded proteins for degradation, thereby maintaining proteostasis. It plays critical roles in neurodegenerative diseases, such as Alzheimer's and Parkinson's, by regulating tau and α-synuclein aggregation. Recent studies also implicate it in cancer progression, immune regulation, and cellular responses to oxidative stress.
Recombinant KIAA0415 is typically expressed in bacterial or mammalian systems for biochemical studies, enabling researchers to investigate its structure-function relationships, substrate interactions, and enzymatic mechanisms. Its applications span drug discovery efforts aiming to modulate protein degradation pathways, studies of chaperone-mediated autophagy, and exploration of therapeutic strategies for proteinopathies. Despite progress, the full spectrum of its physiological substrates and disease-specific regulatory networks remains under active investigation.
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