| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GGT6 |
| Uniprot No | Q6P531 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-493aa |
| 氨基酸序列 | MERAEEPVVYQKLLPWEPSLESEEEVEEEETSEALVLNPRRHQDSSRNKAGGLPGTWVRVVAALLLLAVGCSLAVRQLQNQGRSTGSLGSVAPPPGGHSHGPGVYHHGAIISPAGRELLVAGGNVVDAGVGAALCLAVVHPHATGLGAMFWGLFHDSSSGNSTALTSGPAQTLAPGLGLPAALPTLHLLHARFGRLPWPRLLVGPTTLAQEGFLVDTPLARALVARGTEGLCPLLCHADGTPLGAGARATNPQLAAVLRSAALAPTSDLAGDALLSLLAGDLGVEVPSAVPRPTLEPAEQLPVPQGILFTTPSPSAGPELLALLEAALRSGAPIPDPCPPFLQTAVSPESSALAAVDSSGSVLLLTSSLNCSFGSAHLSPSTGVLLSNLVAKSTTSAWACPLILRGSLDDTEADVLGLVASGTPDVARAMTHTLLRHLAARPPTQAQHQHQGQQEPTEHPSTCGQGTLLQVAAHTEHAHVSSVPHACCPFQGF |
| 分子量 | 76.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GGT6(γ-谷氨酰转移酶6)蛋白的模拟参考文献示例(实际文献需通过学术数据库确认)：
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1. **文献名称**：*Cloning and Functional Characterization of Recombinant Human GGT6 in a Baculovirus Expression System*
**作者**：Smith A, et al.
**摘要**：本研究利用杆状病毒表达系统成功克隆并表达了重组人GGT6蛋白，发现其在体外表现出γ-谷氨酰转移酶活性，并揭示了其在谷胱甘肽代谢中的潜在作用。
2. **文献名称**：*Structural Insights into Human GGT6: Crystal Structure and Substrate Specificity Analysis*
**作者**：Zhang L, et al.
**摘要**：通过X射线晶体学解析了重组人GGT6的三维结构，结合酶动力学实验，阐明了其底物结合位点的独特特征，提示其与GGT家族其他成员的差异功能。
3. **文献名称**：*GGT6 Expression in Cancer: Recombinant Protein-Based Mechanistic Study*
**作者**：Chen H, et al.
**摘要**：利用重组人GGT6蛋白研究其在肿瘤细胞中的过表达现象，发现其通过调节氧化应激途径促进癌细胞存活，为靶向治疗提供了依据。
4. **文献名称**：*Comparative Analysis of GGT Isoenzymes: Recombinant GGT6 Exhibits Unique pH-Dependent Activity*
**作者**：Wang Y, et al.
**摘要**：通过重组表达多种GGT同工酶(包括GGT6)，对比其酶学性质，发现GGT6在酸性环境中的活性显著高于其他同工酶，提示其可能的生理病理意义。
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**说明**：以上为模拟内容，实际文献需通过PubMed、Google Scholar或Web of Science等平台检索确认。推荐使用关键词“recombinant human GGT6”、“GGT6 function”或“gamma-glutamyltransferase 6”进行查询。
Recombinant human gamma-glutamyltransferase 6 (GGT6) is a protein engineered to study the enzymological and physiological roles of the GGT family. GGT6. a member of the gamma-glutamyltransferase enzyme family, catalyzes the transfer of gamma-glutamyl groups from glutathione and other substrates, playing a critical role in cellular redox balance, detoxification, and amino acid metabolism. While GGT1. the most studied isoform, is widely expressed in kidneys and the biliary system, GGT6 exhibits restricted expression, primarily detected in reproductive tissues, the nervous system, and certain cancers, suggesting tissue-specific regulatory functions.
The production of recombinant GGT6 via bacterial or mammalian expression systems enables detailed investigation of its structural and catalytic properties. Unlike other GGT isoforms, GGT6 may display unique substrate preferences or regulatory mechanisms, though its biological significance remains less understood. Research focuses on its potential involvement in pathologies such as tumor progression (via oxidative stress modulation) or neurodegenerative disorders. However, challenges in protein solubility and post-translational modifications during recombinant production require optimized expression strategies.
Studying recombinant GGT6 aids in elucidating isoform-specific contributions to glutathione homeostasis and disease pathways, offering insights for therapeutic targeting or biomarker development. Ongoing work seeks to clarify its physiological relevance and interaction networks within specialized cellular environments.
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