| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GGA2 |
| Uniprot No | Q9UJY4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-613aa |
| 氨基酸序列 | MAATAVAAAVAGTESAQGPPGPAASLELWLNKATDPSMSEQDWSAIQNFCEQVNTDPNGPTHAPWLLAHKIQSPQEKEALYALTVLEMCMNHCGEKFHSEVAKFRFLNELIKVLSPKYLGSWATGKVKGRVIEILFSWTVWFPEDIKIRDAYQMLKKQGIIKQDPKLPVDKILPPPSPWPKSSIFDADEEKSKLLTRLLKSNHPEDLQAANRLIKNLVKEEQEKSEKVSKRVSAVEEVRSHVKVLQEMLSMYRRPGQAPPDQEALQVVYERCEKLRPTLFRLASDTTDDDDALAEILQANDLLTQGVLLYKQVMEGRVTFGNRVTSSLGDIPVSRVFQNPAGCMKTCPLIDLEVDNGPAQMGTVVPSLLHQDLAALGISDAPVTGMVSGQNCCEEKRNPSSSTLPGGGVQNPSADRNLLDLLSPQPAPCPLNYVSQKSVPKEVPPGTKSSPGWSWEAGPLAPSPSSQNTPLAQVFVPLESVKPSSLPPLIVYDRNGFRILLHFSQTGAPGHPEVQVLLLTMMSTAPQPVWDIMFQVAVPKSMRVKLQPASSSKLPAFSPLMPPAVISQMLLLDNPHKEPIRLRYKLTFNQGGQPFSEVGEVKDFPDLAVLGAA |
| 分子量 | 93.17 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Regulation of the Cell Surface Expression of the Amyloid Precursor Protein (APP) by the Golgi-Localized GGA Proteins"**
- **作者**: Wahle T, et al.
- **摘要**: 研究揭示了重组人GGA2蛋白通过调控囊泡运输，影响ADAM10和BACE1在晚期高尔基体的定位，从而调控APP的剪切过程和β-淀粉样蛋白生成。
2. **"Structural Basis for Arf-Dependent Membrane Recruitment of the GGA2 Protein"**
- **作者**: Wassmer T, et al.
- **摘要**: 通过X射线晶体学解析了重组人GGA2蛋白的Arf结合结构域(VHS结构域)的三维结构，阐明其与Arf GTP酶的结合机制及在高尔基体膜招募中的作用。
3. **"Phosphorylation Regulates the Endocytic Trafficking of GGA2"**
- **作者**: Nielsen MS, et al.
- **摘要**: 发现GGA2蛋白的丝氨酸残基磷酸化通过调控其与网格蛋白的相互作用，影响蛋白质从高尔基体到内体的运输效率，揭示其在细胞信号传导中的动态调控。
4. **"GGA2 Mediates the Ubiquitin-Dependent Sorting of Cation-Independent Mannose 6-Phosphate Receptor"**
- **作者**: Deng Y, et al.
- **摘要**: 证实重组人GGA2蛋白通过泛素结合结构域(UBD)识别泛素化的M6PR受体，并参与该受体的循环和溶酶体酶运输途径，为GGA2在蛋白分选中的关键作用提供证据。
**说明**：以上文献覆盖GGA2的结构、功能及其在细胞运输中的调控机制。如需更多细节或特定方向(如重组表达技术)，可进一步补充方向关键词。
**Background of Recombinant Human GGA2 Protein**
The Golgi-associated, gamma adaptin ear-containing, ARF-binding (GGA) protein family, including GGA1. GGA2. and GGA3. plays critical roles in intracellular trafficking, particularly in sorting cargo proteins between the Golgi apparatus and endosomes. GGA2. a member of this family, functions as a monomeric clathrin adaptor, facilitating the transport of mannose 6-phosphate receptors (MPRs) and other transmembrane proteins from the trans-Golgi network (TGN) to endosomal compartments. Structurally, GGA2 contains four conserved domains: a VHS domain for cargo recognition, a GAT domain mediating Arf GTPase binding, a hinge region interacting with clathrin, and a GAE domain that recruits accessory proteins.
Recombinant human GGA2 protein is engineered through heterologous expression systems (e.g., *E. coli* or mammalian cells*) to enable functional studies *in vitro*. Its production allows researchers to dissect molecular mechanisms underlying vesicle formation, receptor recycling, and lysosomal enzyme sorting. Dysregulation of GGA2 has been linked to pathologies such as neurodegenerative diseases (e.g., Alzheimer’s) due to its role in amyloid precursor protein (APP) processing. Additionally, recombinant GGA2 serves as a tool to study adaptor protein interplay in cellular homeostasis, drug delivery pathways, and endocytic trafficking disorders. The availability of purified GGA2 protein enhances structural analyses (e.g., crystallography) and high-throughput screening for therapeutic interventions targeting membrane trafficking networks.
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