| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GGA1 |
| Uniprot No | Q9UJY5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-639aa |
| 氨基酸序列 | MEPAMEPETL EARINRATNP LNKELDWASI NGFCEQLNED FEGPPLATRL LAHKIQSPQE WEAIQALTVL ETCMKSCGKR FHDEVGKFRF LNELIKVVSP KYLGSRTSEK VKNKILELLY SWTVGLPEEV KIAEAYQMLK KQGIVKSDPK LPDDTTFPLP PPRPKNVIFE DEEKSKMLAR LLKSSHPEDL RAANKLIKEM VQEDQKRMEK ISKRVNAIEE VNNNVKLLTE MVMSHSQGGA AAGSSEDLMK ELYQRCERMR PTLFRLASDT EDNDEALAEI LQANDNLTQV INLYKQLVRG EEVNGDATAG SIPGSTSALL DLSGLDLPPA GTTYPAMPTR PGEQASPEQP SASVSLLDDE LMSLGLSDPT PPSGPSLDGT GWNSFQSSDA TEPPAPALAQ APSMESRPPA QTSLPASSGL DDLDLLGKTL LQQSLPPESQ QVRWEKQQPT PRLTLRDLQN KSSSCSSPSS SATSLLHTVS PEPPRPPQQP VPTELSLASI TVPLESIKPS NILPVTVYDQ HGFRILFHFA RDPLPGRSDV LVVVVSMLST APQPIRNIVF QSAVPKVMKV KLQPPSGTEL PAFNPIVHPS AITQVLLLAN PQKEKVRLRY KLTFTMGDQT YNEMGDVDQF PPPETWGSL |
| 分子量 | 70.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GGA1蛋白的3篇代表性文献：
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1. **文献名称**：*The GGA proteins: adaptors on the move*
**作者**：Bonifacino JS
**摘要**：综述GGA蛋白家族(包括GGA1)的结构与功能，着重于其作为接头蛋白在跨高尔基体网络(TGN)至内体的货物运输中的作用，涉及重组GGA1蛋白的VHS结构域如何识别特定货物分子的机制。
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2. **文献名称**：*Structural basis for recognition of acidic-cluster dileucine sequence by GGA1*
**作者**：Shiba T, Kametaka S, Kawasaki M et al.
**摘要**：通过X射线晶体学解析重组人GGA1的VHS结构域与靶向肽复合物的结构，阐明其识别含有酸性氨基酸簇-双亮氨酸(ACLL)基序的货物蛋白(如甘露糖6磷酸受体)的分子机制。
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3. **文献名称**：*GGA proteins mediate the recycling pathway of memapsin 2 (BACE)*
**作者**：He X, Zhu G, Koelsch G et al.
**摘要**：研究重组人GGA1蛋白在阿尔茨海默病相关蛋白BACE(β-分泌酶)循环通路中的作用，证实GGA1通过结合BACE的胞内结构域调控其在高尔基体与内体间的再循环过程。
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**备注**：GGA1研究多聚焦于其结构域(VHS、GAT、GAE)在囊泡运输中的功能，重组蛋白常被用于解析互作机制。如需实验细节推荐结合具体数据库(如PubMed)按关键词筛选。
Recombinant human GGA1 (Golgi-localized, gamma-ear-containing, ARF-binding protein 1) is a multidomain adaptor protein involved in intracellular trafficking, particularly in sorting cargo molecules between the Golgi apparatus and endosomes/lysosomes. Belonging to the GGA family, GGA1 facilitates clathrin-coated vesicle formation by recognizing sorting signals on transmembrane proteins (e.g., mannose-6-phosphate receptors) via its VHS domain. Its modular structure includes a VHS domain for cargo recognition, a GAT domain interacting with ARF GTPases and ubiquitin, a hinge region recruiting clathrin, and a GGA ear domain binding accessory proteins like rabaptin-5.
GGA1’s function is critical for maintaining cellular homeostasis, affecting processes like receptor recycling, protein degradation, and lysosomal biogenesis. Dysregulation of GGA1 has been linked to neurodegenerative diseases (e.g., Alzheimer’s) and cancer. Recombinant GGA1 protein, typically produced in bacterial or mammalian expression systems, enables in vitro studies of its structure, interactions, and regulatory mechanisms. Researchers use it to explore post-translational modifications (e.g., phosphorylation), ligand binding kinetics, and the impact of mutations on trafficking efficiency. Its recombinant form is also instrumental in high-throughput drug screening targeting GGA1-related pathways. Studies often employ truncation mutants to dissect domain-specific functions, advancing our understanding of vesicular transport pathologies.
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