| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GFI1 |
| Uniprot No | Q99684 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-422aa |
| 氨基酸序列 | MPRSFLVKSK KAHSYHQPRS PGPDYSLRLE NVPAPSRADS TSNAGGAKAE PRDRLSPESQ LTEAPDRASA SPDSCEGSVC ERSSEFEDFW RPPSPSASPA SEKSMCPSLD EAQPFPLPFK PYSWSGLAGS DLRHLVQSYR PCGALERGAG LGLFCEPAPE PGHPAALYGP KRAAGGAGAG APGSCSAGAG ATAGPGLGLY GDFGSAAAGL YERPTAAAGL LYPERGHGLH ADKGAGVKVE SELLCTRLLL GGGSYKCIKC SKVFSTPHGL EVHVRRSHSG TRPFACEMCG KTFGHAVSLE QHKAVHSQER SFDCKICGKS FKRSSTLSTH LLIHSDTRPY PCQYCGKRFH QKSDMKKHTF IHTGEKPHKC QVCGKAFSQS SNLITHSRKH TGFKPFGCDL CGKGFQRKVD LRRHRETQHG LK |
| 分子量 | 45.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人GFI1蛋白的参考文献及其摘要概括：
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1. **文献名称**: *"Production and characterization of recombinant human GFI1 protein for transcriptional studies"*
**作者**: Smith, J.R., et al.
**摘要**: 该研究利用大肠杆菌表达系统成功表达并纯化了带有His标签的重组人GFI1蛋白，通过EMSA实验验证了其与DNA靶序列的结合能力，并发现其通过N端锌指结构域介导转录抑制功能。
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2. **文献名称**: *"Functional analysis of GFI1 in hematopoietic differentiation using recombinant protein delivery"*
**作者**: Zhao, L., & Phelan, M.
**摘要**: 作者通过哺乳动物细胞(HEK293)制备了重组GFI1蛋白，利用脂质体将其导入造血干细胞，发现GFI1显著抑制髓系分化相关基因表达，揭示了其在造血调控中的关键作用。
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3. **文献名称**: *"Structural insights into GFI1-DNA interaction via recombinant protein crystallography"*
**作者**: Tanaka, K., et al.
**摘要**: 研究团队通过昆虫细胞表达系统获得高纯度GFI1蛋白，解析了其与DNA复合物的晶体结构，阐明了锌指结构域与特定碱基的结合模式，为开发GFI1靶向药物提供结构基础。
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4. **文献名称**: *"Development of a GFI1-based luciferase reporter assay using recombinant protein"*
**作者**: Dee, M.J., & Spooncer, E.
**摘要**: 构建了依赖GFI1蛋白的荧光素酶报告基因系统，利用重组GFI1验证其对靶启动子的调控活性，证明其在白血病细胞中通过招募共抑制因子抑制肿瘤抑制基因。
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(注：上述文献为示例性内容，实际文献需通过数据库检索确认。)
**Background of Recombinant Human GFI1 Protein**
The Growth Factor Independent 1 (GFI1) protein is a zinc finger-containing transcriptional repressor critical in regulating cellular differentiation, proliferation, and apoptosis. It plays pivotal roles in hematopoiesis, immune cell development, and neurogenesis. Structurally, GFI1 contains an N-terminal SNAG repression domain and six C-terminal zinc finger motifs, enabling DNA binding and interaction with chromatin-modifying complexes like histone deacetylases (HDACs). Dysregulation of GFI1 is linked to malignancies, including leukemia, and neurological disorders, underscoring its importance in maintaining cellular homeostasis.
Recombinant human GFI1 protein, produced via bacterial or mammalian expression systems, retains the functional domains necessary for in vitro studies. It is widely utilized to investigate GFI1’s molecular mechanisms in gene regulation, its interplay with signaling pathways (e.g., Notch, Wnt), and its role in disease pathogenesis. Researchers employ this protein in chromatin immunoprecipitation (ChIP), electrophoretic mobility shift assays (EMSAs), and cell-based assays to dissect its transcriptional networks. Additionally, recombinant GFI1 serves as a tool for screening potential therapeutic agents targeting GFI1-related disorders. Its availability accelerates studies on hematopoietic stem cell biology, cancer biology, and neural development, offering insights into therapeutic strategies for GFI1-associated diseases.
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