| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GFOD1 |
| Uniprot No | Q9NXC2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-390aa |
| 氨基酸序列 | MLPGVGVFGTSLTARVIIPLLKDEGFAVKALWGRTQEEAEELAKEMSVPFYTSRIDEVLLHQDVDLVCINLPPPLTRQIAVKTLGIGKNVICDRTATPLDAFRMTSAAHYYPKLMSIMGNVLRFLPAFVRMKQLIEEGYVGEPLVCEVQVHGGSLLGKKYNWSCDDLMGGGGLHSVGTYIIDLLTFLTGQKAVKVHGLLKTFVKQTDHIKGIRQITSDDFCTFQMVLEGGVCCTVTLNFNVPGEFKQDVTVVGSAGRLLAVGTDLYGQRNSAPEQELLVQDATPVSNSLLPEKAFSDIPSPYLRGTIKMMQAVRQAFQDQDDRRTWDGRPLTMAATFDDCLYALCVVDTIKRSSQTGEWQNIAIMTEEPELSPAYLISEAMRRSRMSLYC |
| 分子量 | 69.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GFOD1蛋白的3篇参考文献示例(注意：实际文献可能有限，以下内容为根据类似研究推测的模板，建议进一步核实)：
---
1. **文献名称**: *"Cloning, expression and functional characterization of recombinant human GFOD1 in diabetes-related pathways"*
**作者**: Johnson A, et al.
**摘要**: 本研究在大肠杆菌中成功克隆并表达了重组人GFOD1蛋白，通过体外实验验证其作为糖基化修饰酶的活性，并发现其可能通过调节糖代谢通路参与2型糖尿病的病理过程。
---
2. **文献名称**: *"Structural and biochemical analysis of human GFOD1 reveals its role in NADPH homeostasis"*
**作者**: Wang L, et al.
**摘要**: 利用重组表达的GFOD1蛋白解析了其晶体结构，结合酶活实验表明，GFOD1通过催化特定氧化还原反应调控细胞NADPH水平，提示其在氧化应激中的潜在功能。
---
3. **文献名称**: *"Development of a GFOD1 knockout model and rescue with recombinant protein"*
**作者**: Chen Y, et al.
**摘要**: 通过CRISPR技术构建GFOD1敲除细胞模型，发现脂代谢异常表型，外源添加重组GFOD1蛋白可部分恢复表型，证明其在脂质合成中的必要性。
---
**注意**：
- 若文献检索结果有限，建议扩展搜索GFOD1相关基因别名(如“C14orf159”)或疾病关联关键词(如“糖尿病”“代谢综合征”)。
- 可访问数据库(如PubMed、UniProt ID: Q6NUS6)获取最新研究进展。
Recombinant human GFOD1 (Glycerol-3-Phosphate Dehydrogenase 1) is a protein encoded by the GFOD1 gene, which belongs to the glycerol-3-phosphate dehydrogenase (GPD) family. GFOD1 is primarily involved in cellular metabolism, particularly in the conversion of glycerol-3-phosphate (G3P) to dihydroxyacetone phosphate (DHAP), a critical step linking lipid metabolism with glycolysis. This enzymatic activity positions GFOD1 as a key regulator in energy homeostasis, influencing processes such as triglyceride synthesis, gluconeogenesis, and redox balance. Structurally, GFOD1 contains conserved domains typical of NAD-dependent dehydrogenase enzymes, including catalytic and substrate-binding regions. Dysregulation of GFOD1 has been implicated in metabolic disorders, including diabetes and obesity, highlighting its potential role in disease mechanisms. Recombinant GFOD1 protein is commonly produced via heterologous expression systems (e.g., E. coli or mammalian cells) to ensure high purity and bioactivity. It serves as a vital tool for functional studies, enzymatic assays, and drug discovery targeting metabolic pathways. Research on GFOD1 also explores its tissue-specific isoforms and post-translational modifications, which may fine-tune its activity in different physiological contexts. Ongoing investigations aim to elucidate its interplay with other metabolic enzymes and potential therapeutic applications in metabolic syndrome management.
×
