| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GGCX |
| Uniprot No | P38435 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 533-629aa |
| 氨基酸序列 | ADFPGLHLENFVSEDLGNTSIQLLQGEVTVELVAEQKNQTLREGEKMQLPAGEYHKVYTTSPSPSCYMYVYVNTTELALEQDLAYLQELKEKVENGS |
| 分子量 | 36.41 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GGCX蛋白的3-4篇代表性文献的简要列举(注：文献为假设性示例，实际引用需核实原文)：
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1. **文献名称**：*Expression and functional characterization of recombinant human gamma-glutamyl carboxylase*
**作者**：Berkner KL, Pudota BN
**摘要**：研究通过HEK293细胞表达重组人GGCX蛋白，并证明其在维生素K存在下对靶蛋白(如凝血因子)的羧化活性，揭示酶促反应对辅因子的依赖性。
2. **文献名称**：*Purification and kinetic analysis of recombinant human gamma-glutamyl carboxylase*
**作者**：Tie JK, Jin DY, Stafford DW
**摘要**：开发了重组人GGCX的高效纯化方法，通过酶动力学实验阐明其底物结合特性及对维生素K环氧化物的催化效率，为药物靶点研究提供基础。
3. **文献名称**：*Structural insights into human γ-glutamyl carboxylase: A molecular modeling and mutagenesis study*
**作者**：Mutucumarana VP, et al.
**摘要**：基于重组表达的GGCX蛋白，结合定点突变和分子模拟，鉴定出多个关键功能域，揭示其在羧化反应中的构象变化机制。
4. **文献名称**：*Recombinant expression of human GGCX in insect cells and its role in vitamin K-dependent protein carboxylation*
**作者**：Sugiura I, et al.
**摘要**：利用杆状病毒-昆虫细胞系统表达重组GGCX，验证其与膜结合特性及在维生素K代谢中的作用，为研究遗传性GGCX缺陷疾病提供模型。
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如需实际文献，建议通过PubMed或Web of Science以关键词“recombinant human GGCX”检索最新研究。
Recombinant human GGCX (Gamma-glutamyl carboxylase) is a key enzyme involved in the post-translational modification of vitamin K-dependent proteins. GGCX catalyzes the carboxylation of specific glutamic acid residues in these proteins, a critical step required for their biological activity, such as blood coagulation (factors II, VII, IX, X), bone metabolism (osteocalcin), and vascular health (Matrix Gla protein). This process relies on vitamin K as a cofactor, which is oxidized during the reaction and must be recycled for sustained enzymatic activity.
Mutations in the GGCX gene are associated with bleeding disorders, skeletal abnormalities, or ectopic calcification due to impaired carboxylation. Recombinant GGCX protein, typically produced in mammalian or insect cell systems, enables detailed study of its structure-function relationships, enzymatic mechanisms, and interactions with substrates or inhibitors. Researchers use it to model diseases, screen potential therapeutics (e.g., anticoagulants), and explore its role in conditions like cardiovascular calcification or osteoporosis. Its recombinant form provides a standardized tool for in vitro assays, overcoming challenges related to protein solubility and stability in native purification. Current studies also focus on modulating GGCX activity to balance pro-/anti-coagulant pathways or regulate tissue mineralization.
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