| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM49A |
| Uniprot No | Q9H0Q0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-323aa |
| 氨基酸序列 | MGNLLKVLTREIENYPHFFLDFENAQPTEGEREIWNQISAVLQDSESILADLQAYKGAGPEIRDAIQNPNDIQLQEKAWNAVCPLVVRLKRFYEFSIRLEKALQSLLESLTCPPYTPTQHLEREQALAKEFAEILHFTLRFDELKMRNPAIQNDFSYYRRTISRNRINNMHLDIENEVNNEMANRMSLFYAEATPMLKTLSNATMHFVSENKTLPIENTTDCLSTMTSVCKVMLETPEYRSRFTSEETLMFCMRVMVGVIILYDHVHPVGAFCKTSKIDMKGCIKVLKEQAPDSVEGLLNALRFTTKHLNDESTSKQIRAMLQ |
| 分子量 | 61.93 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FAM49A蛋白的3篇假设性文献示例(供参考，需通过学术数据库核实具体信息)：
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1. **文献名称**：*FAM49A suppresses cell migration by antagonizing RAB protein activity*
**作者**：Klinkermann et al. (2021)
**摘要**：研究揭示了FAM49A通过与RAB家族蛋白相互作用，调控细胞骨架重塑，抑制肿瘤细胞的迁移和侵袭能力，并证明了重组FAM49A蛋白在体外实验中的功能验证。
2. **文献名称**：*Structural and functional analysis of human FAM49A in innate immunity*
**作者**：Chen et al. (2022)
**摘要**：通过X射线晶体学解析了重组人FAM49A蛋白的三维结构，发现其C端结构域可能参与免疫信号调控，实验表明其通过结合RIG-I通路成分抑制病毒诱导的干扰素产生。
3. **文献名称**：*FAM49A regulates macrophage polarization via ROS signaling*
**作者**：Zhang et al. (2019)
**摘要**：利用重组FAM49A蛋白在巨噬细胞中的功能研究，证明其通过调节活性氧(ROS)水平影响巨噬细胞的M1/M2极化，进而参与炎症反应和代谢调控。
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**提示**：上述文献为示例，实际研究中可查阅 **PubMed** 或 **Google Scholar** 以关键词“FAM49A protein”、“recombinant human FAM49A”检索最新成果。
**Background of Recombinant Human FAM49A Protein**
Recombinant human FAM49A protein is a genetically engineered form of the endogenous FAM49A (Family with sequence similarity 49 member A), a protein encoded by the *FAM49A* gene. FAM49A, also known as CYRI-A (CYFIP-related Rac1 interactor A), is a cytosolic protein implicated in regulating cellular processes such as cytoskeletal reorganization, vesicular trafficking, and immune responses. It interacts with Rac1 GTPase, modulating its activity to influence cell migration, membrane ruffling, and autophagy. Dysregulation of FAM49A has been linked to cancers, infectious diseases, and inflammatory conditions, though its precise mechanistic roles remain under investigation.
The recombinant form is typically expressed in bacterial or mammalian systems, enabling functional studies of FAM49A in vitro and in vivo. Research focuses on its role in diseases: for example, FAM49A suppresses invasive tumor behavior in certain cancers but may promote pro-inflammatory signaling in immune cells. Its structural features, including conserved domains and phosphorylation sites, are critical for interactions with signaling partners. Current gaps include understanding post-translational modifications, tissue-specific functions, and therapeutic potential. Recombinant FAM49A protein serves as a vital tool to unravel these aspects, offering insights into targeted therapies for cancer, immune disorders, or infections like *Salmonella*, where FAM49A influences host-pathogen interactions.
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