| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM48A |
| Uniprot No | Q8NEM7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-779aa |
| 氨基酸序列 | MQQALELALDRAEYVIESARQRPPKRKYLSSGRKSVFQKLYDLYIEECEKEPEVKKLRRNVNLLEKLVMQETLSCLVVNLYPGNEGYSLMLRGKNGSDSETIRLPYEEGELLEYLDAEELPPILVDLLEKSQVNIFHCGCVIAEIRDYRQSSNMKSPGYQSRHILLRPTMQTLICDVHSITSDNHKWTQEDKLLLESQLILATAEPLCLDPSIAVTCTANRLLYNKQKMNTRPMKRCFKRYSRSSLNRQQDLSHCPPPPQLRLLDFLQKRKERKAGQHYDLKISKAGNCVDMWKRSPCNLAIPSEVDVEKYAKVEKSIKSDDSQPTVWPAHDVKDDYVFECEAGTQYQKTKLTILQSLGDPLYYGKIQPCKADEESDSQMSPSHSSTDDHSNWFIIGSKTDAERVVNQYQELVQNEAKCPVKMSHSSSGSASLSQVSPGKETDQTETVSVQSSVLGKGVKHRPPPIKLPSSSGNSSSGNYFTPQQTSSFLKSPTPPPSSKPSSIPRKSSVDLNQVSMLSPAALSPASSSQRTTATQVMANSAGLNFINVVGSVCGAQALMSGSNPMLGCNTGAITPAGINLSGLLPSGGLLPNALPSAMQAASQAGVPFGLKNTSSLRPLNLLQLPGGSLIFNTLQQQQQQLSQFTPQQPQQPTTCSPQQPGEQGSEQGSTSQEQALSAQQAAVINLTGVGSFMQSQAAVLSQLGSVENRPEQSLPQQRFQLSSAFQQQQQQIQQLRFLQHQMAMAAAAAQTAQLHHHRHTGSQSKSKMKRGTPTTPKF |
| 分子量 | 112.09 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FAM48A蛋白的参考文献示例(部分内容基于已有研究方向的推测，实际文献可能需要通过学术数据库验证)：
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1. **文献名称**：*FAM48A interacts with SUMO3 to regulate transcriptional repression and stress response*
**作者**：Gupta R, et al.
**摘要**：本研究通过重组表达人FAM48A蛋白，验证其与SUMO3的直接相互作用，并发现该互作在细胞应激反应(如氧化应激)中调控转录抑制复合物的活性，影响靶基因表达。
2. **文献名称**：*Structural characterization of human FAM48A reveals a conserved SPRY domain critical for mRNA processing*
**作者**：Chen L, et al.
**摘要**：文章解析了重组FAM48A蛋白的晶体结构，发现其N端SPRY结构域介导与RNA结合蛋白的相互作用，提示其在mRNA剪接或稳定性调控中的潜在作用。
3. **文献名称**：*FAM48A suppresses tumorigenesis by modulating autophagy-lysosomal pathways in breast cancer*
**作者**：Wang X, et al.
**摘要**：利用重组FAM48A蛋白进行功能实验，发现其在乳腺癌细胞中通过激活自噬途径抑制肿瘤增殖，并揭示其表达水平与患者预后呈正相关。
4. **文献名称**：*High-throughput identification of FAM48A-binding kinases using recombinant protein arrays*
**作者**：Kim S, et al.
**摘要**：通过重组FAM48A蛋白文库筛选，鉴定出多个互作激酶(如AKT1)，并证明FAM48A可能通过磷酸化修饰参与细胞周期调控。
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**注意**：以上为基于FAM48A已知研究方向(如SUMO化、SPRY结构域功能、自噬调控等)的模拟参考文献。如需真实文献，建议使用PubMed或Web of Science搜索“recombinant FAM48A”或“FAM48A function”等关键词获取。
Recombinant human FAM48A protein, also known as SUPT20H or CCDC111. is a genetically engineered version of the endogenous protein encoded by the FAM48A gene located on chromosome 16q24.3. This protein belongs to the FAM48 family and functions as a nuclear factor involved in transcriptional regulation, RNA splicing, and cellular stress responses. Studies suggest its interaction with components of the transcription elongation complex, potentially influencing histone modification and chromatin remodeling processes.
FAM48A is notably implicated in SUMOylation-dependent pathways, facilitating post-translational modifications critical for protein stability and activity. Recombinant FAM48A is typically produced using expression systems like Escherichia coli or mammalian cell lines to ensure proper folding and post-translational modifications. Its purified form enables in vitro studies to dissect molecular mechanisms, such as its role in modulating the p53 tumor suppressor pathway or interacting with stress granule proteins under oxidative stress.
Current research links FAM48A dysregulation to pathologies including cancers, neurodegenerative disorders, and autoimmune diseases. However, its precise physiological roles remain under investigation. Recombinant variants, often tagged for purification (e.g., His-tag), are pivotal in structural analysis, antibody production, and high-throughput screening for therapeutic targeting. Despite progress, further functional studies are needed to elucidate its interactome and therapeutic potential in disease models.
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